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Human TNF RI/TNFRSF1A ELISA Kit (Colorimetric)

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ELISA: Human TNF RI/TNFRSF1A ELISA Kit (Colorimetric) [NBP2-60166] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Human TNF RI/TNFRSF1A ELISA Kit (Colorimetric) [NBP2-60166] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, tissue homogenates and urine
Standard Curve Range
78.125 pg/mL - 5000 pg/mL
Sensitivity
65 pg/mL

Order Details

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Human TNF RI/TNFRSF1A ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of human TNFsR- I . No significant cross-reactivity or interference between human TNFsR-I and analogues was observed.
Standard Curve Range
78.125 pg/mL - 5000 pg/mL
Sensitivity
65 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 10
Intra-Assay
%CV < 8
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
TNFRSF1A

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Adhesive Strip (For 96 wells)
  2. Assay plate (12 x 8 coated Microwells)
  3. Biotin-antibody (100 x concentrate)
  4. Biotin-antibody Diluent
  5. HRP-avidin (100 x concentrate)
  6. HRP-avidin Diluent
  7. Instruction manual
  8. Sample Diluent
  9. Standard (Freeze dried)
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (25 x concentrate)

Alternate Names for Human TNF RI/TNFRSF1A ELISA Kit (Colorimetric)

  • CD120a antigen
  • CD120a
  • FPF
  • p55
  • p55-R
  • p60
  • TNF RI
  • TNFARMGC19588
  • TNF-R
  • TNF-R1
  • TNFR1TBP1
  • TNFR55
  • TNF-R55
  • TNFR60
  • TNFRI
  • TNF-RI
  • TNF-R-I
  • TNFR-I
  • TNFRSF1A
  • tumor necrosis factor binding protein 1
  • Tumor necrosis factor receptor 1
  • tumor necrosis factor receptor 1A isoform beta
  • tumor necrosis factor receptor superfamily member 1A
  • tumor necrosis factor receptor superfamily, member 1A
  • tumor necrosis factor receptor type 1
  • Tumor necrosis factor receptor type I
  • tumor necrosis factor-alpha receptor

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TNFsR- I has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNFsR-I present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFsR-I is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFsR-I bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol TNFRSF1A