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Thrombospondin-3 Antibody

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Immunohistochemistry-Paraffin: Thrombospondin-3 Antibody [NBP1-36996] - Staining of Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Po, Bv, CaSpecies Glossary
Applications ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Thrombospondin-3 Antibody Summary

Immunogen
Peptide with sequence CNDTVPEDFEPFRRQ corresponding to C-Terminus according to NP_009043.1.
Epitope
C Terminus
Predicted Species
Mouse (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
THBS3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1 - 2 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Preliminary experiments gave an approx. 50 kDa band in human lung and in lysates of A549 after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 104 kDa band according to NP_009043.1. The 50 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human colon shows endothelial cell staining of a blood vessel.
Publications
Read Publication using NBP1-36996.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Thrombospondin-3 Antibody

  • MGC119565
  • THBS3
  • thrombospondin 3
  • Thrombospondin3
  • Thrombospondin-3
  • TSP3
  • TSP3MGC119564

Background

THBS3 is a gene that codes for an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions and has two isoforms, with lengths of 956 and 836 amino acids and weights of approximately 104 and 91 kDa respectively. Current research is being done on diseases and disorders related to this gene including osteosarcoma, lung cancer, breast cancer, retinitis, malaria, neuronitis, and echinococcosis. THBS3 has also been shown to have interactions with THBS2, THBS1, THBS4, FURIN, and PDGFB in pathways such as the focal adhesion, inflammatory response, signal transduction, signaling by PDGF, phagosome, ECM-receptor interaction, and malaria pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Thrombospondin-3 Antibody (NBP1-36996)(1)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol THBS3
Entrez
Uniprot