![Western Blot: TGF-alpha Antibody (SPM542) - Azide and BSA Free [NBP2-34418] - analysis of lysate from HEK293 cells (30ug) using anti-TGF alpha antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/TGF-alpha-Antibody-SPM542-Azide-and-BSA-Free-Western-Blot-NBP2-34418-img0001.jpg "Western Blot: TGF-alpha Antibody (SPM542) - Azide and BSA Free [NBP2-34418] - analysis of lysate from HEK293 cells (30ug) using anti-TGF alpha antibody. Image from verified customer review.")
| Reactivity | Hu, Rb, ZeSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | SPM542 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 350 | NBP2-34418AF350 | |||
| Alexa Fluor 532 | NBP2-34418AF532 | |||
| Alexa Fluor 594 | NBP2-34418AF594 | |||
| Alexa Fluor 750 | NBP2-34418AF750 | |||
| DyLight 594 | NBP2-34418DL594 | |||
| FITC | NBP2-34418F | |||
| Unconjugated | NBP2-34371-0.1mg | |||
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34371). Antibody with azide - store at 2 to 8C. |
| Immunogen | A 10-amino acid synthetic peptide (aa34-43; PPVAAAVVSH) from human TGF-alpha (Uniprot: P01135) |
| Localization | Cytoplasmic and Extracellular (secreted) |
| Specificity | This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this monoclonal antibody is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. |
| Isotype | IgM Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TGFA |
| Purity | Protein L or PEG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. The staining with this MAb is completely blocked by the peptide used for raising this antibody. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein L or PEG purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for TGF-alpha Antibody (NBP2-34418)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | TGFA |
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