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TGF-alpha Antibody (P/T1) [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, Rb, ZeSpecies Glossary
Applications Flow, ICC/IF, IHC, CyTOF-ready
Clone
P/T1
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-34702AF350
Alexa Fluor 405
NBP2-34702AF405
Alexa Fluor 488
NBP2-34702AF488
Alexa Fluor 532
NBP2-34702AF532
Alexa Fluor 594
NBP2-34702AF594
Alexa Fluor 647
NBP2-34702AF647
Alexa Fluor 700
NBP2-34702AF700
Alexa Fluor 750
NBP2-34702AF750
Allophycocyanin
NBP2-34702APC
Biotin
NBP2-34702B
DyLight 350
NBP2-34702UV
DyLight 405
NBP2-34702V
DyLight 488
NBP2-34702G
DyLight 550
NBP2-34702R
DyLight 594
NBP2-34702DL594
DyLight 650
NBP2-34702C
DyLight 680
NBP2-34702FR
DyLight 755
NBP2-34702IR
FITC
NBP2-34702F
HRP
NBP2-34702H
Janelia Fluor 525
NBP2-34702JF525
Janelia Fluor 549
NBP2-34702JF549
Janelia Fluor 646
NBP2-34702JF646
Janelia Fluor 669
NBP2-34702JF669
PE
NBP2-34702PE
PerCP
NBP2-34702PCP
Unconjugated
NBP2-34299-0.1mg
mFluor Violet 450 SE
NBP2-34702MFV450
mFluor Violet 500 SE
NBP2-34702MFV500
mFluor Violet 610 SE
NBP2-34702MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-34299-0.1mg
Azide and BSA Free
NBP2-34702-0.1mg
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TGF-alpha Antibody (P/T1) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
A 10-amino acid synthetic peptide (aa34-43; PPVAAAVVSH) from human TGF-alpha (Uniprot: P01135)
Localization
Cytoplasmic and Extracellular (secreted)
Specificity
This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this monoclonal antibody is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TGFA
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for TGF-alpha Antibody (P/T1) [CoraFluor™ 1]

  • protransforming growth factor alpha
  • TFGA
  • TGFA
  • TGFalpha
  • TGF-alpha
  • transforming growth factor, alpha
  • transforming growth factor-alpha

Background

Transforming growth factor alpha (TGFalpha) is an acid- and heat-stable 50 amino acid protein originally found in rodents and humans. TGFalpha is 33% homologous at the amino acid level to epidermal growth factor (EGF). TGFalpha binds to the EGF receptor, mediates tyrosine phosphorylation of the receptor and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor beta. TGFalpha is secreted by a variety of transformed cells and tumors, embryonic cells and some normal adult cells. TGFalpha bioactivity has been found in the urine of cancer patients. It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Amphiregulin
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VEGF [HRP]
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N/A VEGF [HRP]
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[Unconjugated]
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[Unconjugated]
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c-Myc Antibody
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SOS1 Antibody
(SOS-01) - BSA Free
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Applications: CyTOF-ready, ICC, IHC, ICFlow

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TGFA