Reactivity | MuSpecies Glossary |
Applications | Flow, ICC/IF, IHC, IP |
Clone | H57-597 |
Clonality | Monoclonal |
Host | Rabbit |
Conjugate | PE |
Additional Information | Recombinant Monoclonal Antibody |
Immunogen | Hamsters were immunised with affinity purified DO-11.10 TCR, and produced detectable anti-TCR antibodies which were judged by the ability of the anti-sera to immunoprecipitate TCR from surface labelled DO-11.10 T cell hybridomas. Hamster 57 was selected for hybridisation, and B cell hybridomas were produced using polyethylene glycol fusion of hamster splenic B cells to the mouse myeloma variant. Selected mAb were screened after subsequent culturing for their ability to immunoprecipitate TCR from detergent lysates of surface-radiolabelled murine T cell hybridomas. |
Epitope | H57-597 is directed to an epitope within the FG loop of the C region of TCR beta chain. |
Specificity | mAb H57-597 reacts with surface receptors on all alpha-beta TCR bearing cells, but does not react with receptors on gamma-delta T cells. |
Isotype | IgG Lambda |
Clonality | Monoclonal |
Host | Rabbit |
Gene | Tcrb |
Purity | Protein A purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Optimal dilution of this antibody should be experimentally determined. |
Storage | Store at 4C in the dark. |
Buffer | PBS |
Preservative | 0.05% Sodium Azide |
Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for TCR beta Antibody (NBP3-20118PE)Find related products by research area.
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Gene Symbol | Tcrb |