![Western Blot: TCF3/E2A Antibody [NBP2-20585] - Sample (30 ug of whole cell lysate) A: BCL-1 7. 5% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/TCF3-E2A-Antibody-Western-Blot-NBP2-20585-img0001.jpg "Western Blot: TCF3/E2A Antibody [NBP2-20585] - Sample (30 ug of whole cell lysate) A: BCL-1 7. 5% SDS PAGE gel, diluted at 1:2000.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human TCF3. The exact sequence is proprietary. |
| Localization | Nucleus |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TCF3 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 68 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Western Blot beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Western_Blot_5100.jpg)
![Chromatin Immunoprecipitation (ChIP) beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Chromatin_Immunoprecipitation_11251.jpg)
![Simple Western beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/betaCatenin_AF1329_Simple_Western_17134.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for TCF-3/E2A Antibody (NBP2-20585)Find related products by research area.
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![Immunoprecipitation: TCF-3/E2A Antibody [NBP2-20585] - Jurkat whole cell extract A. 35 ug Jurkat whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of TCF3 protein by 4 ug TCF3 antibody 7.5 % SDS-PAGE The immunoprecipitated TCF3 protein was detected by TCF3 antibody diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/TCF-3-E2A-Antibody-Immunoprecipitation-NBP2-20585-img0004.jpg "Immunoprecipitation: TCF-3/E2A Antibody [NBP2-20585] - Jurkat whole cell extract A. 35 ug Jurkat whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of TCF3 protein by 4 ug TCF3 antibody 7.5 % SDS-PAGE The immunoprecipitated TCF3 protein was detected by TCF3 antibody diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]")
![Immunocytochemistry/ Immunofluorescence: TCF-3/E2A Antibody [NBP2-20585] - TCF-3/E2A antibody detects TCF3 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TCF3 stained by TCF-3/E2A antibody (NBP2-20585) diluted at 1:600.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-20585_rabbit-polyclonal-tcf-3-e2a-antibody-9102024205688.jpg "Immunocytochemistry/ Immunofluorescence: TCF-3/E2A Antibody [NBP2-20585] - TCF-3/E2A antibody detects TCF3 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TCF3 stained by TCF-3/E2A antibody (NBP2-20585) diluted at 1:600.Blue: Fluoroshield with DAPI .")
![Immunohistochemistry-Paraffin: TCF-3/E2A Antibody [NBP2-20585] - TCF-3/E2A antibody detects TCF3 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.TCF3 stained by TCF-3/E2A antibody (NBP2-20585) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-20585_rabbit-polyclonal-tcf-3-e2a-antibody-91020242011507.jpg "Immunohistochemistry-Paraffin: TCF-3/E2A Antibody [NBP2-20585] - TCF-3/E2A antibody detects TCF3 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.TCF3 stained by TCF-3/E2A antibody (NBP2-20585) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: TCF-3/E2A Antibody [NBP2-20585] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCF-3/E2A antibody (NBP2-20585) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-20585_rabbit-polyclonal-tcf-3-e2a-antibody-91020242063331.jpg "Western Blot: TCF-3/E2A Antibody [NBP2-20585] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCF-3/E2A antibody (NBP2-20585) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")