Home » TATA Element Modulatory Factor 1 » TATA Element Modulatory Factor 1 Lysates » TATA Element Modulatory Factor 1 Overexpression Lysate
Novus Biologicals products are now on bio-techne.com

TATA Element Modulatory Factor 1 Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: TATA Element Modulatory Factor 1 Overexpression Lysate (Adult Normal) [NBP2-10384] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TATA Element Modulatory Factor 1.
Western Blot: TATA Element Modulatory Factor 1 Overexpression Lysate (Adult Normal) [NBP2-10384] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TATA Element ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

TATA Element Modulatory Factor 1 Overexpression Lysate Summary

Description

TATA Element Modulatory Factor 1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC220590

Accession#: NM_007114

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
TMF1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
122.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for TATA Element Modulatory Factor 1 Overexpression Lysate

  • TATA element modulatory factor 1

Background

Potential coactivator of the androgen receptor. Mediates STAT3 degradation. May play critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. This protein binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Androgen R/NR3C4
[p Ser213, p Ser210] ...
NB100-56603
Western Blot Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) - BSA Free
(14 Publications)
Species: Ca, Hu, Mu, Pm, Rt
Applications: IHC, IHC-P, WB
NCOA4 Antibody
(1B7)
H00008031-M04
ELISA NCOA4 Antibody (1B7)
Immunocytochemistry/ Immunofluorescence NCOA4 Antibody (1B7)
Western Blot NCOA4 Antibody (1B7)
(5 Publications)
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
Ran Antibody
(8D1A6) - BSA Free
NBP2-61832
ELISA Ran Antibody (8D1A6) - BSA Free
Flow Cytometry Ran Antibody (8D1A6) - BSA Free
Immunocytochemistry/ Immunofluorescence Ran Antibody (8D1A6) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
HIC5/TGFB1I1
Antibody [Unconjugate
AF5626
Western Blot HIC5/TGFB1I1 Antibody [Unconjugated]
Immunocytochemistry HIC5/TGFB1I1 Antibody [Unconjugated]
Species: Hu
Applications: ICC, WB
Ferredoxin
Reductase Antibody
NBP2-38530
Western Blot Ferredoxin Reductase Antibody
Immunohistochemistry-Paraffin Ferredoxin Reductase Antibody
Immunohistochemistry-Paraffin Ferredoxin Reductase Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
AKR1B1 Antibody
NBP1-89146
Immunohistochemistry-Paraffin AKR1B1 Antibody
Immunohistochemistry-Paraffin AKR1B1 Antibody
Immunohistochemistry-Paraffin AKR1B1 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Amphiregulin
Antibody
AF262
Neutralization Amphiregulin Antibody
Immunocytochemistry Amphiregulin Antibody
Immunohistochemistry Amphiregulin Antibody
(1 Review)
(35 Publications)
Species: Hu
Applications: ICC, IHC, Neut, WB
ARA54 Antibody
NBP1-89021
Immunohistochemistry-Paraffin ARA54 Antibody
Western Blot ARA54 Antibody
Western Blot ARA54 Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
RAB6A Antibody
NBP1-33110
Immunocytochemistry/ Immunofluorescence RAB6A Antibody
Western Blot RAB6A Antibody
Western Blot RAB6A Antibody
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
GOLPH3 Antibody
NBP2-75515
Flow Cytometry GOLPH3 Antibody
Immunocytochemistry/ Immunofluorescence GOLPH3 Antibody
Immunocytochemistry/ Immunofluorescence GOLPH3 Antibody
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC-P, WB
Human, Mouse, Rat
RelA/NFkB p65 ELISA K ...
NBP2-29661
ELISA Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric)
ELISA Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric)
ELISA Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric)
(95 Publications)
Species: Hu, Mu, Rt
Applications: ELISA
Brg1 Antibody
[Unconjugated]
AF5738
Western Blot Brg1 Antibody [Unconjugated]
Immunocytochemistry Brg1 Antibody [Unconjugated]
Immunocytochemistry Brg1 Antibody [Unconjugated]
(1 Review)
(3 Publications)
Species: Hu
Applications: ICC, KO, WB
BRM Antibody
NBP3-13283
Immunohistochemistry-Paraffin BRM Antibody
Immunohistochemistry-Paraffin BRM Antibody
Immunocytochemistry/ Immunofluorescence BRM Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
KMT3B/NSD1
Antibody (4F1)
H00064324-M08
Western Blot KMT3B/NSD1 Antibody (4F1)
(3 Publications)
Species: Hu
Applications: ELISA, IP, WB
Supervillin
Antibody
NBP1-90363
Immunohistochemistry-Paraffin Supervillin Antibody
Immunohistochemistry-Paraffin Supervillin Antibody
Western Blot Supervillin Antibody
(2 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
STAT3 Antibody
(232209) [Unconjugate
MAB1799
Knockout Validated STAT3 Antibody (232209) [Unconjugated]
Western Blot STAT3 Antibody (232209) [Unconjugated]
Knockout Validated STAT3 Antibody (232209) [Unconjugated]
(23 Publications)
Species: Hu, Mu, Rt
Applications: ICC, IP, ICFlow, KO, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(820 Publications)
Species: Hu
Applications: BA
DBT Antibody
NBP1-89522
Immunocytochemistry/ Immunofluorescence DBT Antibody
Immunohistochemistry-Paraffin DBT Antibody
Immunohistochemistry-Paraffin DBT Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Fer Antibody - BSA
Free
NBP1-20089
Western Blot Fer Antibody - BSA Free
Western Blot Fer Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence Fer Antibody - BSA Free
Species: Hu
Applications: ICC/IF, KD, WB
WNK1 Antibody
[Unconjugated]
AF2849
Western Blot WNK1 Antibody [Unconjugated]
(5 Publications)
Species: Mu, Rt
Applications: WB

Publications for TATA Element Modulatory Factor 1 Lysate (NBP2-10384) (0)

There are no publications for TATA Element Modulatory Factor 1 Lysate (NBP2-10384).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TATA Element Modulatory Factor 1 Lysate (NBP2-10384) (0)

There are no reviews for TATA Element Modulatory Factor 1 Lysate (NBP2-10384). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TATA Element Modulatory Factor 1 Lysate (NBP2-10384) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional TATA Element Modulatory Factor 1 Products

Research Areas for TATA Element Modulatory Factor 1 Lysate (NBP2-10384)

Find related products by research area.

Blogs on TATA Element Modulatory Factor 1

There are no specific blogs for TATA Element Modulatory Factor 1, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TATA Element Modulatory Factor 1 Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol TMF1