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HIC5/TGFB1I1 Antibody [Unconjugated]

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Western blot shows lysates of MCF-7 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HIC5/TGFB1I1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5626) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HIC5/TGFB1I1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of MCF-7 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HIC5/TGFB1I1 Antigen Affinity-purified ...read more
HIC5/TGFB1I1 was detected in immersion fixed PC-3 human prostate cancer cell line, unstimulated (upper panel) or stimulated with 10 ng/mL Recombinant Human BMP-4 (Catalog # 314-BP; lower panel), using Goat Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF5626-SP
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HIC5/TGFB1I1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human HIC5
Val184-Gly288
Accession # O43294
Specificity
Detects endogenous human HIC5 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TGFB1I1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HIC5/TGFB1I1 Antibody [Unconjugated]

  • Androgen receptor coactivator 55 kDa protein
  • androgen receptor coactivator ARA55
  • Androgen receptor-associated protein of 55 kDa
  • ARA55
  • ARA55Hydrogen peroxide-inducible clone 5 protein
  • HIC5
  • HIC-5
  • TGFB1I1
  • transforming growth factor beta 1 induced transcript 1
  • transforming growth factor beta-1-induced transcript 1 protein
  • TSC-5

Background

HIC5 (Hydrogen peroxide inducible clone 5; also ARA55) is a 50-55 kDa group III member of the LIM domain family of proteins. It is expressed primarily in smooth muscle, platelets and myoepithelium. It resides in both cytoplasm and nucleus, and performs multiple functions. It associates with focal adhesions, binds to the nuclear matrix, and serves as a coactivator for the glucocorticoid and androgen receptors. Human HIC5 is 461 amino acids (aa) in length. It contains four Leu:Asp‑rich motifs (aa 1‑215) and four LIM domains (aa 226‑461). LIM domains, either individually, or in combination, perform the majority of functions. LIM4 binds to the nuclear matrix, LIMs 3 and 4 are coactivators, and LIMs 2 and 3 bind to focal adhesions. HIC5 is induced by TGF beta and by hydrogen peroxide.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TGFB1I1
Uniprot