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TAP2 Antibody (998524) [Unconjugated]

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Western blot shows lysates of human tonsil tissue and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TAP2 Monoclonal Antibody (Catalog # MAB2432) followed ...read more
TAP2 was detected in immersion fixed U-251 MG human glioblastoma cell line (left panel) and HL-60 human acute promyelocytic leukemia cell line (right panel, negative control) using Mouse Anti-Human TAP2 Monoclonal ...read more
TAP2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human TAP2 Monoclonal Antibody (Catalog # MAB2432) at 8 µg/mL for 1 hour at room temperature followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clone
998524
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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TAP2 Antibody (998524) [Unconjugated] Summary

Immunogen
Synthetic peptide containing human TAP2
Accession # Q03519
Specificity
Detects human TAP2 in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB2432 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TAP2 Antibody (998524) [Unconjugated]

  • ABC18
  • ABCB3
  • ABCB3ABC18
  • antigen peptide transporter 2
  • APT2
  • ATP-binding cassette, sub-family B (MDR/TAP), member 3
  • D6S217E
  • MHC 2
  • Peptide transporter involved in antigen processing 2
  • Peptide transporter PSF2
  • Peptide transporter TAP2
  • PSF2
  • PSF2PSF-2
  • Really interesting new gene 11 protein
  • RING11
  • RING11ATP-binding cassette sub-family B member 3
  • TAP2
  • transporter 2, ABC (ATP binding cassette)
  • transporter 2, ATP-binding cassette, sub-family B (MDR/TAP)
  • Y1

Background

TAP2 transports antigens from the cytoplasm to the endoplasmic reticulum, where they can bind to MHC class 1 molecules.  It does this translocation by selecting peptides based on both their length and their sequence.  The peptide selection occurs during the first step of the translocation process.  Transmembrane segments of TAP form a pore in the membrane and the peptide binding site is formed by the cytosolic component of the pore.  Inherited deficiency in the TAP transporter can lead to recurrent respiratory bacterial infections due to HLA class I deficiency (BLS1).
  1. Nijenjuis M., Hämmerling Journal of immunology; 1996 Dec 15;157(12):5467-5477
  2. de la Salle H, Hanau D, Fricker D, Urlacher A, Kelly A, Salamero J, Powis SH, Donato L, Bausinger H, Laforet M, (1994), Science, Jul 8; 265(5169):237-241.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TAP2 Antibody (MAB2432) (0)

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Review for TAP2 Antibody (MAB2432) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB2432:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Yongkang Yang
WB Human 11/24/2022
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Summary

ApplicationWestern Blot
Sample Testedbreast cancer MCF7 cells,HEK293 cell lysate
SpeciesHuman
LotCLYL01181

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Yongkang Yang
11/24/2022
Application: WB
Species: Human

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