Why dose your Y5R antibodies produces 2 different molecular weight products in human tissues or human cell lysates? NB100-1538 ab probes to 50KDa protein while the rabbit Y5R ab probes to 40-42KDa protein?

Name: NPY5R Antibody
Brand: Novus Biologicals
SKU: NB100-1538
Price: 399 USD

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Accepted Answer

NPY5R is a glycosylated protein. This can cause it to run at varying molecular weights depending on the cell line and Western blot conditions. The 48 kDa band represents the protein in it's unglycosylated form whereas the higher molecular weights above 50 kDa are from blots generated in conditions when the protein is in its glycosylated form.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Rt, Ha, Mu, CaSpecies Glossary
Applications	WB, ELISA, IHC, IP
Clonality	Polyclonal
Host	Goat
Conjugate	Unconjugated
Concentration	0.5 mg/ml

Immunogen	Peptide with sequence C-GLSNKENRLEEN corresponding to internal region according to NP_006165.1.
Predicted Species	Mouse (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	NPY5R
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at -20C. Avoid freeze-thaw cycles.
Buffer	Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative	0.02% Sodium Azide
Concentration	0.5 mg/ml
Purity	Immunogen affinity purified

Publications using NB100-1538	Applications	Species
Gheller BJ, Blum JE, Merritt EK et al. Peptide YY (PYY) Is Expressed in Human Skeletal Muscle Tissue and Expanding Human Muscle Progenitor Cells Front Physiol 2019-03-05 [PMID: 30890955] (WB, Human)	WB	Human
Hsieh YS, Kuo MH, Chen PN, Kuo DY. The Identification of Neuropeptide Y Receptor Subtype Involved in Phenylpropanolamine-induced Increase in Oxidative Stress and Appetite Suppression Neuromolecular Med 2012-11-20 [PMID: 23179670] (WB, Rat)	WB	Rat
Kuo DY, Chen PN, Yu CH et al. Involvement of neuropeptide Y Y1 receptor in the regulation of amphetamine-mediated appetite suppression Neuropharmacology 2012-06-22 [PMID: 22732442] (WB, Rat)	WB	Rat
Igura K, Haider HK, Ahmed RP et al. Neuropeptide Y and Neuropeptide Y Y5 Receptor Interaction Restores Impaired Growth Potential of Aging Bone Marrow Stromal Cells. Rejuvenation Res. 2011-05-19 [PMID: 21595512]
Badman MK, Flier JS. The gut and energy balance: visceral allies in the obesity wars. Science 2005-03-25 [PMID: 15790843]

NPY5R Antibody

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Bioinformatics

Gene Symbol	NPY5R
Entrez	4889(Human)
Uniprot	Q15761(Human)