Immunohistochemistry-Paraffin: TAF15 Antibody [NB100-566] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti- TAFII68 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunoprecipitation: TAF15 Antibody [NB100-566] - Detection of human TAFII68 on HeLa whole cell lysate using NB100-566. TAFII68 was IPed with rabbit anti-TAFII68 antibodies NB100-566, NB100-567, NB100-568, and another ...read more
A synthetic peptide which maps to a region between residues 175 and 225 of human TAFII68 using the numbering given in Swiss-Prot entry Q92804 (GeneID 8148).
Specificity
NB100-566 is specific for human TAFII-68 protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TAF15
Purity
Immunogen affinity purified
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Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. In some cases, the antibody may be diluted further than indicated. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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