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T-bet/TBX21 Antibody [mFluor Violet 500 SE]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
mFluor Violet 500 SE

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-41235AF350
Alexa Fluor 405
NBP2-41235AF405
Alexa Fluor 488
NBP2-41235AF488
Alexa Fluor 532
NBP2-41235AF532
Alexa Fluor 594
NBP2-41235AF594
Alexa Fluor 647
NBP2-41235AF647
Alexa Fluor 700
NBP2-41235AF700
Alexa Fluor 750
NBP2-41235AF750
Biotin
NBP2-41235B
CoraFluor 1
NBP2-41235CL1
DyLight 350
NBP2-41235UV
DyLight 405
NBP2-41235V
DyLight 488
NBP2-41235G
DyLight 550
NBP2-41235R
DyLight 594
NBP2-41235DL594
DyLight 650
NBP2-41235C
DyLight 680
NBP2-41235FR
DyLight 755
NBP2-41235IR
FITC
NBP2-41235F
HRP
NBP2-41235H
Janelia Fluor 525
NBP2-41235JF525
Janelia Fluor 549
NBP2-41235JF549
Janelia Fluor 585
NBP2-41235JF585
Janelia Fluor 635
NBP2-41235JF635
Janelia Fluor 646
NBP2-41235JF646
Janelia Fluor 669
NBP2-41235JF669
PerCP
NBP2-41235PCP
Unconjugated
NBP2-41235
mFluor Violet 450 SE
NBP2-41235MFV450
mFluor Violet 610 SE
NBP2-41235MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-41235
BSA Free
NBP2-41235
BSA Free
NBP2-41235-0.025mg
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T-bet/TBX21 Antibody [mFluor Violet 500 SE] Summary

Immunogen
Antibody was raised against a 19 amino acid peptide near the carboxy terminus of human TBX2. The immunogen is located within the last 50 amino acids of TBX21 . Amino Acid Squence: APSPFDKEAEGQFYNYFPN
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TBX21
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Peptide affinity purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for T-bet/TBX21 Antibody [mFluor Violet 500 SE]

  • Tbet
  • T-bet
  • TBETT-PET
  • TBLYM
  • TBLYMT-cell-specific T-box transcription factor T-bet
  • T-box 21
  • T-box expressed in T cells
  • T-box protein 21
  • T-box transcription factor TBX21
  • TBX21
  • T-PET
  • Transcription factor TBLYM

Background

Tbx21 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for T-bet/TBX21 Antibody (NBP2-41235MFV500). (Showing 1 - 1 of 1 FAQ).

  1. Do you make a Tbx21 ab for FACS for porcine?
    • We have not tested our Tbx21 antibodies for porcine, however you might like to test NBP1-43298 as part of our Innovators Reward Program. This is gained from testing a product on a species or application that we have not yet tested. I have carried out a blast to check the % homology between the pig protein and human protein, this is 94% and our lab recommends use if the % homology is 85% or above.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TBX21