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Rat Syndecan-2/CD362 ELISA Kit (Colorimetric)

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ELISA: Rat Syndecan-2/CD362 ELISA Kit (Colorimetric) [NBP2-76584] - Samples were spiked with high concentrations of Rat Syndecan-2/CD362 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat Syndecan-2/CD362 ELISA Kit (Colorimetric) [NBP2-76584] - Samples were spiked with high concentrations of Rat Syndecan-2/CD362 and diluted with Reference Standard & Sample Diluent to produce samples with ...read more
ELISA: Rat Syndecan-2/CD362 ELISA Kit (Colorimetric) [NBP2-76584] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.313 - 20ng/mL
Sensitivity
0.188ng/mL

Order Details

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Rat Syndecan-2/CD362 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat HSPG in samples. No significant cross-reactivity or interference between Rat HSPG and analogues was observed.
Standard Curve Range
0.313 - 20ng/mL
Sensitivity
0.188ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.81
Intra-Assay
CV% < 5.16
Spike Recovery
86-104%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
SDC2

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat Syndecan-2/CD362 ELISA Kit (Colorimetric)

  • CD362 antigen
  • CD362
  • Fibroglycan
  • heparan sulfate proteoglycan 1, cell surface-associated
  • Heparan sulfate proteoglycan core protein
  • HSPG
  • HSPG1
  • HSPG1syndecan proteoglycan 2
  • SDC2
  • SYND2
  • SYND2cell surface-associated heparan sulfate proteoglycan 1
  • syndecan 2
  • Syndecan2
  • Syndecan-2

Background

Proteoglycans are macromolecules consisting of a variety of core proteins with covalently attached one or several polysaccharide chains of the glycosaminoglycan type (heparan sulphate, heparin, chondroitin sulphate, dermatan sulphate or keratan sulphate). At least two forms of basement membrane heparan sulphate proteoglycan (HSPG) have been identified. One with a large core protein (> 400 kD) and one with a small core protein (30 kD). The large HSPG is probably the most abundant basement membrane proteoglycan. It is located predominantly in the lamina lucida, where it forms clustered aggregates and interacts with other basement membrane components to form the matrix. In addition, it also plays a critical role in attachment of cells to the basal membrane via integrin receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

  SDS
  Rat Syndecan-2/CD362 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol SDC2