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Syndecan-1/CD138 Antibody (359103) [Janelia Fluor® 635]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, CyTOF-ready, Dual ISH-IHC, ICC/IF
Clone
359103
Clonality
Monoclonal
Host
Rat
Conjugate
Janelia Fluor 635

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Syndecan-1/CD138 Antibody (359103) [Janelia Fluor® 635] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Syndecan-1/CD138
Gln18-Glu251
Accession # NP_002988
Specificity
Detects human Syndecan-1 in direct ELISAs. In direct ELISAs, this antibody shows approximately 60% cross-reactivity with recombinant mouse (rm) Syndecan‑1 and no cross-reactivity with recombinant human (rh) Syndecan-2, rhSyndecan-3, or rmSyndecan-4.
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
SDC1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Dual RNAscope ISH-IHC
  • Flow Cytometry
  • Immunocytochemistry
  • Immunohistochemistry
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Syndecan-1/CD138 Antibody (359103) [Janelia Fluor® 635]

  • CD138 antigen
  • CD138
  • SDC
  • SDC1
  • SYND1heparan sulfate proteoglycan fibroblast growth factor receptor
  • syndecan 1
  • syndecan proteoglycan 1
  • syndecan
  • Syndecan1
  • Syndecan-1

Background

CD138, also known as Syndecan-1, is a transmembrane heparin sulfate proteoglycan that binds type I collagen. The relative molecular mass of CD138 is found to vary between 20-95 kDa due to the presence of a variable number of GAG modifications on each CD138 molecule. This protein appears to mediate binding of cells to the extracellular matrix. Among mature tissues, CD138 is expressed on simple and stratified epithelia, endothelial cells, and fibroblasts. (2,3) While stratified keratinocytes express CD138, their malignant transformation suppresses CD138 expression. Wound repair processes alter patterns of CD138 expression. Among hematopoietic cells, CD138 is expressed by immunoglobulin-negative pre-B cells in the bone marrow, immature B cells and by terminally differentiated plasma cells in lymphoid organs. CD138 expression has not been observed on mature, circulating peripheral B lymphocytes, however. (4-7) CD138 is also strongly expressed by multiple myeloma-derived cell lines and elevated levels of CD138 are present in the serum of patients with multiple myeloma, a negative prognostic indicator for the disease. (8)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SDC1