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SYK Antibody (720402) [Unconjugated] - (SH2D2 Domain)

 
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Western blot shows lysates of EL-4 mouse lymphoblast cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SYK (SH2D2 Domain) Monoclonal Antibody (Catalog # MAB7166) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for SYK (SH2D2 Domain) at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of EL-4 mouse lymphoblast cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SYK (SH2D2 Domain) Monoclonal Antibody (Catalog # ...read more
Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SYK at approximately 66 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human SYK ...read more
Simple Western lane view shows lysates of EL‑4 mouse lymphoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for SYK at approximately 66 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human SYK ...read more
Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line and SYK knockout THP-1 cell line (KO). Nitrocellulose membrane was probed with 2 µg/mL of Mouse Anti-Human SYK (SH2D2 Domain) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, KO
Clone
720402
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB7166-SP
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SYK Antibody (720402) [Unconjugated] - (SH2D2 Domain) Summary

Immunogen
E. coli-derived recombinant human SYK (SH2D2 Domain)
Trp168-Cys259
Accession # P43405
Specificity
Detects human SYK (SH2D2 Domain) in direct ELISAs. In direct ELISAs, 100% cross-reactivity with the SH2D2 domain of recombinant human (rh) ZAP70 is observed and no cross-reactivity with the SH2 domain of rhCSK is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SYK
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Knockout Validated
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SYK Antibody (720402) [Unconjugated] - (SH2D2 Domain)

  • DKFZp313N1010
  • EC 2.7.10
  • EC 2.7.10.2
  • FLJ25043
  • spleen tyrosine kinaseFLJ37489
  • SYK
  • tyrosine-protein kinase SYK

Background

SYK (spleen tyrosine kinase) is a 70-80 kDa cytoplasmic non-receptor protein tyrosine kinase of the protein kinase superfamily and the SYK/ZAP-70 subfamily of proteins. The 635 amino acid (aa) human SYK contains two SH2 domains (aa 14-106 and aa 168-259), and one protein kinase domain (aa 371-631). A splicing variant produces a second isoform lacking aa 371-631. Within the second SH2 domain (SH2D2), human SYK shares 94% aa sequence identity with mouse and rat SYK. SYK is widely expressed in hematopoietic cells (notably B lymphocytes), where it couples immunoglobulin receptors to downstream events, such as proliferation, differentiation, and phagocytosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SYK
Uniprot