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SWAP70 Antibody

Images

 
Orthogonal Strategies: Western Blot: SWAP70 Antibody [NBP1-82979] - Analysis in human cell lines A-431 and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.
Western Blot: SWAP70 Antibody [NBP1-82979] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: SWAP70 Antibody [NBP1-82979] - Human dendritic cells were pulsed with FITC-labeled zymosan (zymosan-FITC; green in merge)(pink arrowheads) and latex beads (yellow arrowhead) ...read more
Immunohistochemistry-Paraffin: SWAP70 Antibody [NBP1-82979] - Staining of human lymph node shows strong cytoplasmic positivity in germinal center cells.
Western Blot: SWAP70 Antibody [NBP1-82979] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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SWAP70 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KLEEAASRAAEEEKKRLQTQVELQARFSTELEREKLIRQQMEEQVAQKSSELEQYLQRVRELEDMYLKLQEALEDERQARQDEETVRKLQAR
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SWAP70
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence validated for ICC/IF from a verified customer review.
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
SWAP70 Protein (NBP1-82979PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-82979 in the following applications:

Publications
Read Publications using
NBP1-82979 in the following applications:

  • ICC
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SWAP70 Antibody

  • KIAA0640SWAP-70FLJ39540
  • SWAP switching B-cell complex 70kDa subunit
  • SWAP70
  • switch-associated protein 70

Background

Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SWAP70 Antibody (NBP1-82979)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC, WB.


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ICC
(1)
WB
(1)
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Human
(1)
Mouse
(1)
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Review for SWAP70 Antibody (NBP1-82979) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human and Mouse.

Reviews using NBP1-82979:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Human and Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry SWAP70 NBP1-82979
Enlarge
5
reviewed by:
Maksim Baranov
ICC Human and Mouse 11/23/2016
View

Summary

ApplicationImmunocytochemistry
Sample TestedBlood-derived monocytes,Raw 246.7 cells
SpeciesHuman and Mouse

Comments

CommentsFor immunocytochemistry the 1:200 dilution was used
For Western Blot the 1:500 dilution was used

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SWAP70 Antibody (NBP1-82979) (0)

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Maksim Baranov
11/23/2016
Application: ICC
Species: Human and Mouse

Bioinformatics

Gene Symbol SWAP70