Orthogonal Strategies: Western Blot: SWAP70 Antibody [NBP1-82979] - Analysis in human cell lines A-431 and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.
Western Blot: SWAP70 Antibody [NBP1-82979] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: SWAP70 Antibody [NBP1-82979] - Human dendritic cells were pulsed with FITC-labeled zymosan (zymosan-FITC; green in merge)(pink arrowheads) and latex beads (yellow arrowhead) ...read more
Immunohistochemistry-Paraffin: SWAP70 Antibody [NBP1-82979] - Staining of human lymph node shows strong cytoplasmic positivity in germinal center cells.
Western Blot: SWAP70 Antibody [NBP1-82979] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
This antibody was developed against Recombinant Protein corresponding to amino acids: KLEEAASRAAEEEKKRLQTQVELQARFSTELEREKLIRQQMEEQVAQKSSELEQYLQRVRELEDMYLKLQEALEDERQARQDEETVRKLQAR
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SWAP70
Purity
Immunogen affinity purified
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Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified
Alternate Names for SWAP70 Antibody
KIAA0640SWAP-70FLJ39540
SWAP switching B-cell complex 70kDa subunit
SWAP70
switch-associated protein 70
Background
Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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