Novus Biologicals products are now on bio-techne.com

DEF6 Antibody (OTI3F9)

Images

 
Western Blot: DEF6 Antibody (3F9) [NBP2-46456] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DEF6.
Immunohistochemistry: DEF6 Antibody (3F9) [NBP2-46456] - Analysis of Human endometrium tissue.
Immunohistochemistry: DEF6 Antibody (3F9) [NBP2-46456] - Analysis of Human pancreas tissue.
Immunohistochemistry: DEF6 Antibody (3F9) [NBP2-46456] - Analysis of Human lymphoma tissue.
Immunohistochemistry: DEF6 Antibody (3F9) [NBP2-46456] - Analysis of Human tonsil tissue.
Immunohistochemistry: DEF6 Antibody (3F9) [NBP2-46456] - Analysis of Human bladder tissue.
Immunohistochemistry: DEF6 Antibody (3F9) [NBP2-46456] - Analysis of Human breast tissue.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI3F9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

DEF6 Antibody (OTI3F9) Summary

Immunogen
Full length human recombinant protein of human DEF6(NP_071330) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DEF6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Western Blot 1:2000
Theoretical MW
73.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DEF6 Antibody (OTI3F9)

  • DEF-6
  • differentially expressed in FDCP 6 homolog (mouse)
  • differentially expressed in FDCP 6 homolog
  • IBPdifferentially expressed in FDCP (mouse homolog) 6
  • IRF4-binding protein
  • SLAT
  • SWAP70L
  • SWAP-70-like adaptor protein of T cells

Background

DEF6, or IBP, is a guanine nucleotide exchange factor (GEF) for RAC (MIM 602048) and CDC42 (MIM 116952) that is highly expressed in B and T cells (Gupta et al., 2003 [PubMed 12923183]).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-82979
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
AF6260
Species: Hu
Applications: WB
NB110-68800
Species: Hu, Mu
Applications: ICC/IF, WB
NBP1-85727
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-59690
Species: Am, Av, Fi, Ma, Ze
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IP, WB
NBP1-58359
Species: Hu
Applications: IHC, IHC-P, WB
NB100-41398
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
NB100-91266
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP2-21577
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, WB
H00056731-M02
Species: Hu
Applications: ELISA, ICC/IF, WB
AF594
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, Neut, WB
AF5525
Species: Hu
Applications: IHC, WB
NBP2-67895
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, IP, WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
AF5415
Species: Hu
Applications: ICC, IHC, Simple Western, WB
202-IL
Species: Hu
Applications: BA
NBP1-82018
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NB100-56566
Species: Bv, Hu, Ma, Mu, Po, Rt
Applications: B/N, ChIP, CyTOF-ready, DB, Dual ISH-IHC, ELISA(Cap), ELISA, Flow-CS, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, KD, KO, PAGE, WB

Publications for DEF6 Antibody (NBP2-46456) (0)

There are no publications for DEF6 Antibody (NBP2-46456).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for DEF6 Antibody (NBP2-46456) (0)

There are no reviews for DEF6 Antibody (NBP2-46456). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DEF6 Antibody (NBP2-46456) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DEF6 Antibody (OTI3F9) and receive a gift card or discount.

Bioinformatics

Gene Symbol DEF6