![Western Blot: SUCLA2 Antibody [NBP1-33015] - Sample (30 ug of whole cell lysate) A: Hep G2 B: Molt-4 10% SDS PAGE diluted at 1:1000.](http://images.novusbio.com/fullsize/SUCLA2-Antibody-Western-Blot-NBP1-33015-img0009.jpg "Western Blot: SUCLA2 Antibody [NBP1-33015] - Sample (30 ug of whole cell lysate) A: Hep G2 B: Molt-4 10% SDS PAGE diluted at 1:1000.")
| Reactivity | Hu, Mu, Bv, ChSpecies Glossary |
| Applications | WB, Simple Western, ICC/IF, IHC, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human SUCLA2. The exact sequence is proprietary. |
| Localization | Mitochondrion |
| Predicted Species | Bovine (92%), Chicken (90%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SUCLA2 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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Enlarge |
reviewed by:
zhenqi zhou |
WB | Mouse | 03/12/2019 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for SUCLA2 Antibody (NBP1-33015)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SUCLA2 |
![Immunocytochemistry/Immunofluorescence: SUCLA2 Antibody [NBP1-33015] - SUCLA2 antibody [N2C3] detects SUCLA2 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: SUCLA2 protein stained by SUCLA2 antibody [N2C3] diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/SUCLA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33015-img0012.jpg "Immunocytochemistry/Immunofluorescence: SUCLA2 Antibody [NBP1-33015] - SUCLA2 antibody [N2C3] detects SUCLA2 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: SUCLA2 protein stained by SUCLA2 antibody [N2C3] diluted at 1:500.Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: SUCLA2 Antibody [NBP1-33015] - Mouse stomach. SUCLA2 antibody [N2C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SUCLA2-Antibody-Immunohistochemistry-Paraffin-NBP1-33015-img0010.jpg "Immunohistochemistry-Paraffin: SUCLA2 Antibody [NBP1-33015] - Mouse stomach. SUCLA2 antibody [N2C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: SUCLA2 Antibody [NBP1-33015] - Sample (20 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:20000.](http://images.novusbio.com/fullsize/SUCLA2-Antibody-Western-Blot-NBP1-33015-img0008.jpg "Western Blot: SUCLA2 Antibody [NBP1-33015] - Sample (20 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:20000.")
![Immunohistochemistry-Paraffin: SUCLA2 Antibody [NBP1-33015] - Cal27 xenograft, using SUCLA2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SUCLA2-Antibody-Immunohistochemistry-Paraffin-NBP1-33015-img0007.jpg "Immunohistochemistry-Paraffin: SUCLA2 Antibody [NBP1-33015] - Cal27 xenograft, using SUCLA2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Knockout Validated: SUCLA2 Antibody [NBP1-33015] - Wild-type (WT) and SUCLA2 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SUCLA2 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SUCLA2-Antibody-Western-Blot-NBP1-33015-img0011.jpg "Knockout Validated: SUCLA2 Antibody [NBP1-33015] - Wild-type (WT) and SUCLA2 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SUCLA2 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Simple Western: SUCLA2 Antibody [NBP1-33015] - Simple Western Lane view shows HepG2, Molt-4, U-251-MG whole cell lysate (WCL). A specific band was detected for SUCLA2 antibody (NBP1-33015) at approximately 50 kDa (as indicated) using 10 ug/mL of SUCLA2 antibody. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/SUCLA2-Antibody-Simple-Western-NBP1-33015-img0013.jpg "Simple Western: SUCLA2 Antibody [NBP1-33015] - Simple Western Lane view shows HepG2, Molt-4, U-251-MG whole cell lysate (WCL). A specific band was detected for SUCLA2 antibody (NBP1-33015) at approximately 50 kDa (as indicated) using 10 ug/mL of SUCLA2 antibody. This experiment was conducted under reducing conditions.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
