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SUB1 Antibody

Images

 
Western Blot: SUB1 Antibody [NB100-59774] - Detection of Human PC4 on HeLa whole cell lysate using NB100-59774.
Immunohistochemistry-Paraffin: SUB1 Antibody [NB100-59774] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PC4 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Product Details

Summary
Reactivity Hu, Pm-OrSpecies Glossary
Applications WB, IHC, IP, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

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SUB1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 25 and 75 of human positive cofactor 4 using the numbering given in entry NP_006704.2 (GeneID 10923).
Predicted Species
Orangutan (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SUB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation 2 - 5 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 30992017).
Control
SUB1 Overexpression Lysate
Publications
Read Publications using
NB100-59774 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SUB1 Antibody

  • activated RNA polymerase II transcription cofactor 4
  • activated RNA polymerase II transcriptional coactivator p15
  • MGC102747
  • p15
  • PC4p14P15
  • Positive cofactor 4
  • RPO2TC1
  • SUB1 homolog (S. cerevisiae)
  • SUB1 homolog

Background

PC4 is a multifunctional transcriptional coactivator that plays an important role in transcription, repair, and replication. PC4 interacts with general transcription factors, DNA repair factors, and mRNA processing factors to influence transcription initiation, coactivation, polyadenylation, transcription termination, and transcriptional repression. PC4 has been demonstrated to be a DNA binding protein and a bona fide component of chromatin with chromatin-compacting ability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SUB1 Antibody (NB100-59774)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Chemotaxis.


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Chemotaxis
(1)
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Human
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for SUB1 Antibody (NB100-59774) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SUB1
Entrez
Uniprot