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EXOSC3 Antibody

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Biological Strategies: Western Blot: EXOSC3 Antibody [NBP2-22261] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. NBP2-22261 used for WB at 0.1 ug/ml. ...read more
Immunoprecipitation: EXOSC3 Antibody [NBP2-22261] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22261 used for IP at 6 ug/mg lysate. EXOSC3 was also immunoprecipitated by ...read more
SPT6 Depletion Causes H3K36me3 Redistribution(A) Western blots with indicated antibodies 60 hr post-siSPT6 transfection (versus siLuc nonspecific control). EXOSC3 & H3 profiles shown as loading controls.(B) ...read more

Product Details

Summary
Reactivity Hu, Mu, ChSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

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EXOSC3 Antibody Summary

Immunogen
The immunogen this antibody was made to, maps to a region between residue 225 and 275 of human Exosome Component 3 using the numbering given in entry NP_057126.2 (GeneID 51010).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EXOSC3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 2 - 10 ug/mg
  • Western Blot 1:2000 - 1:10000
Reviewed Applications
Read 1 Review rated 4
using
NBP2-22261 in the following applications:

Publications
Read Publications using
NBP2-22261 in the following applications:

  • WB
    1 publication

Reactivity Notes

Chicken reactivity reported in (PMID: 24842995).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EXOSC3 Antibody

  • bA3J10.7
  • CGI-102
  • exosome complex exonuclease RRP40
  • exosome component 3MGC15120
  • exosome component Rrp40
  • hRrp-40
  • hRrp40p
  • p10Rrp40p
  • Ribosomal RNA-processing protein 40
  • RP11-3J10.8
  • RRP40MGC723

Background

EXOSC3 is a component of the exosome 3'->5' exoribonuclease complex, a complex that degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions. Required for the 3'processing of the 7S pre-RNA to the mature 5.8S rRNA. Ha

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EXOSC3 Antibody (NBP2-22261)(2)

Review for EXOSC3 Antibody (NBP2-22261) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NBP2-22261:
Filter by Applications
WB
(1)
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Other
(1)
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Images Ratings Applications Species Date Details
Western Blot EXOSC3 NBP2-22261
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4
reviewed by:
Hsin Jing-Ping
WB Other 10/18/2013
View

Summary

ApplicationWestern Blot
SpeciesOther

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for EXOSC3 Antibody (NBP2-22261) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on EXOSC3

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Hsin Jing-Ping
10/18/2013
Application: WB
Species: Other

Bioinformatics

Gene Symbol EXOSC3
Uniprot