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STAT3 [p Ser727] Antibody (788335) [Unconjugated]

 (4 Publications)
 (1 Review)
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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IL-6 (Catalog # 206-IL) for 15 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-STAT3 (S727) Monoclonal Antibody (Catalog # MAB4934) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-STAT3 (S727) at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IL-6 (Catalog # 206-IL) for 15 minutes. PVDF membrane was probed with 1 µg/mL ...read more
Daudi human Burkitt's lymphoma cell line was unstimulated (open histogram) or treated with 500 U/mL rhIFN-alpha for 20 minutes (filled histogram) and stained with Mouse anti-Human Phospho-STAT3 (S727) Molyclonal ...read more
Regulation of hepcidin by IL-10 in primary macrophages, showing (a) the effect of IL-10 on Hepcidin mRNA synthesis with and without anti-IL-10 blocking antibody; (b) Western blot of STAT3 phosphorylation 20 minutes ...read more
Regulation of hepcidin by IL-10 in primary macrophages, showing (a) the effect of IL-10 on Hepcidin mRNA synthesis with and without anti-IL-10 blocking antibody; (b) Western blot of STAT3 phosphorylation 20 minutes ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow
Clone
788335
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC4934U-100UG
Alexa Fluor 405
IC4934V-100UG
Alexa Fluor 488
IC4934G-100UG
Alexa Fluor 532
FAB4934X
Alexa Fluor 594
IC4934T-100UG
Alexa Fluor 647
IC4934R-100UG
Alexa Fluor 700
IC4934N-100UG
Alexa Fluor 750
IC4934S-100UG
Allophycocyanin
FAB4934A
Biotin
FAB4934B
CoraFluor 1
FAB4934CL1
DyLight 350
FAB4934D
DyLight 405
FAB4934E
DyLight 488
FAB4934K
DyLight 550
FAB4934L
DyLight 594
FAB4934M
DyLight 650
FAB4934W
DyLight 680
FAB4934Y
DyLight 755
FAB4934Z
FITC
FAB4934F
HRP
FAB4934H
Janelia Fluor 525
FAB4934JF525
Janelia Fluor 549
FAB4934I
Janelia Fluor 646
FAB4934J
Janelia Fluor 669
FAB4934JF669
PE
FAB4934P
PerCP
FAB4934C
mFluor Violet 450 SE
FAB4934MFV450
mFluor Violet 500 SE
FAB4934MFV500
mFluor Violet 610 SE
FAB4934MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB4934-SP
Carrier Free
IC4934V-100UG
Carrier Free
IC4934U-100UG
Carrier Free
IC4934T-100UG
Carrier Free
IC4934S-100UG
Carrier Free
IC4934R-100UG
Carrier Free
IC4934N-100UG
Carrier Free
IC4934G-100UG
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STAT3 [p Ser727] Antibody (788335) [Unconjugated] Summary

Immunogen
Phosphopeptide containing the human STAT3 S727 site
Modification
p Ser727
Specificity
Detects human STAT3 when phosphorylated at S727.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
STAT3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB4934 in the following applications:
Publications
Read Publications using
MAB4934 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STAT3 [p Ser727] Antibody (788335) [Unconjugated]

  • Acute-phase response factor
  • APRFMGC16063
  • DNA-binding protein APRF
  • FLJ20882
  • HIES
  • signal transducer and activator of transcription 3 (acute-phase responsefactor)
  • signal transducer and activator of transcription 3
  • STAT3

Background

Signal Transducer and Activator of Transcription (STAT) proteins are transcription factors activated in response to cytokine, growth factor, or hormone receptor signaling. Janus kinases (JAKs) phosphorylate STAT proteins and induce dimerization. Homo- or heterodimers translocate to the nucleus where they bind to DNA and activate transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for STAT3 Antibody (MAB4934)(4)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Western Blot.


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Western Blot
(3)
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(2)
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(1)
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Review for STAT3 Antibody (MAB4934) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB4934:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot STAT3 MAB4934
Enlarge 		5
reviewed by:
Verified Customer
 WB Human 10/11/2018
View

Summary

ApplicationWestern Blot
Sample TestedHuman breast cancer cell lines
SpeciesHuman
LotCGVC0112041

Product General Protocols

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FAQs for STAT3 Antibody (MAB4934) (0)

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Recent Reviews

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Western Blot STAT3 MAB4934
Enlarge
 Verified Customer
10/11/2018
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol STAT3