![Immunohistochemistry-Paraffin: StAR Antibody (STAR/2140) - Azide and BSA Free [NBP2-75740] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with StAR Mouse Monoclonal Antibody (STAR/2140).](http://images.novusbio.com/fullsize/StAR-Antibody-STAR-2140-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-75740-img0002.jpg "Immunohistochemistry-Paraffin: StAR Antibody (STAR/2140) - Azide and BSA Free [NBP2-75740] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with StAR Mouse Monoclonal Antibody (STAR/2140).")
| Reactivity | HuSpecies Glossary |
| Applications | IHC, MA |
| Clone | STAR/2140 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-75739). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant fragment (around aa 39-108) of human StAR protein (exact sequence is proprietary) (Uniprot: P49675) |
| Localization | Cytoplasmic (Mitochondria) |
| Marker | Leydig Cell Marker |
| Specificity | Steroidogenic Acute Regulatory Protein (STAR) controls the rate-limiting step of steroidegenesis by translocating cholesterol from the outer mitochondrial membrane to the inner membrane where it is later cleaved to pregnenolone. It is primarily present in steroid-producing cells, including Leydig cells in the testis, theca cells and luteal cells in the ovary and adrenal cells in the adrenal cortex. Due to low levels of pregnenolone, seminomas and Leydig cell tumors display no specific STAR staining. Therefore, STAR antibody may assist in differentiating sex cord stromal tumors (SCST), seminomas and embryonal carcinomas. |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | STAR |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 30 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for StAR Antibody (NBP2-75740)Find related products by research area.
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| Gene Symbol | STAR |
![Immunohistochemistry-Paraffin: StAR Antibody (STAR/2140) - Azide and BSA Free [NBP2-75740] - Formalin-fixed, paraffin-embedded human Adrenal Gland stained with StAR Mouse Monoclonal Antibody (STAR/2140).](http://images.novusbio.com/fullsize/StAR-Antibody-STAR-2140-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-75740-img0001.jpg "Immunohistochemistry-Paraffin: StAR Antibody (STAR/2140) - Azide and BSA Free [NBP2-75740] - Formalin-fixed, paraffin-embedded human Adrenal Gland stained with StAR Mouse Monoclonal Antibody (STAR/2140).")
![Protein Array: StAR Antibody (STAR/2140) - Azide and BSA Free [NBP2-75740] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.](http://images.novusbio.com/fullsize/StAR-Antibody-STAR-2140-Azide-and-BSA-Free-Protein-Array-NBP2-75740-img0003.jpg "Protein Array: StAR Antibody (STAR/2140) - Azide and BSA Free [NBP2-75740] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.")
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