KG-1a human acute myelogenous leukemia cell line was stained with Goat Anti-Human ST2/IL-33 R APC-conjugated Polyclonal Antibody (Catalog # FAB5231A, filled histogram) or isotype control antibody (Catalog # IC108A, open ...read more
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ST2/IL-33 R Lys19-Phe328 Accession # BAA02233
Specificity
Detects human ST2/IL-33 R in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL-18 RI, and rhST-7 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL1RL1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for ST2/IL-33R Antibody [Allophycocyanin]
DER4
DER4ST2growth stimulation-expressed
Fit-1
IL-1 R4
IL1R4
IL-1R4
IL-1RL1
IL-33 R
IL33R
IL-33R
interleukin 1 receptor-like 1
interleukin 1 receptor-related protein
interleukin-1 receptor-like 1
Ly84
MGC32623
Protein ST2
ST2
ST2L
ST2V
T1
T1homolog of mouse growth stimulation-expressed
Background
ST2, also known as IL-33R, IL-1 R4 and T1, is an
Interleukin-1 receptor family glycoprotein that contributes to Th2 immune
responses (1, 2). Human ST2 consists of a 310 amino acid (aa) extracellular
domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a
207 aa cytoplasmic domain with an intracellular TIR domain (3, 4). Alternate
splicing of the 120 kDa human ST2 generates a soluble 60 kDa isoform that lacks
the transmembrane and cytoplasmic regions as well as an isoform that
additionally lacks the third Ig‑like domain (4). Within the ECD,
human ST2 shares 68% and 64% aa sequence identity with mouse and rat ST2,
respectively. ST2 is expressed on the surface of mast cells, activated Th2
cells, macrophages, and cardiac myocytes (5‑8). It binds IL-33, a cytokine
that is upregulated by inflammation or mechanical strain in smooth muscle
cells, airway epithelia, keratinocytes, and cardiac fibroblasts (5, 9). IL-33
binding induces the association of ST2 with IL-1R AcP, a shared signaling
subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 10, 11). In
macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by
sequestering the adaptor proteins MyD88 and Mal (7). In addition to its role in
promoting mast cell and Th2 dependent inflammation, ST2 activation enhances
antigen induced hypernociception and protects from atherosclerosis and cardiac
hypertrophy (5, 12‑14). The soluble ST2 isoform is released by activated
Th2 cells and strained cardiac myocytes and is elevated in the serum in
allergic asthma (6, 8, 15). Soluble ST2 functions as a decoy receptor that
blocks IL‑33's ability
to signal through transmembrane ST2 (10, 13‑15).
Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31.
Tominaga, S. et al. (1992) Biochim. Biophys. Acta 1171:215.
Li, H. et al. (2000) Genomics 67:284.
Schmitz, J. et al. (2005) Immunity 23:479.
Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979.
Brint, E.K. et al. (2004) Nat. Immunol. 5:373.
Weinberg, E.O. et al. (2002) Circulation 106:2961.
Sanada S. et al. (2007) J. Clin. Invest. 117:1538.
Palmer, G. et al. (2008) Cytokine 42:358.
Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051.
Verri Jr., W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723.
Miller, A.M. et al. (2008) J. Exp. Med. 205:339.
Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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