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SSRP1 Antibody

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Western Blot: SSRP1 Antibody [NBP1-33235] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SSRP1 antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: SSRP1 Antibody [NBP1-33235] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SSRP1 antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit ...read more
Immunohistochemistry-Paraffin: SSRP1 Antibody [NBP1-33235] - Paraffin-embedded Cal27 Xenograft, using antibody at 1:100 dilution.
Western Blot: SSRP1 Antibody [NBP1-33235] - (30 ug of whole cell lysate) Molt-4 7.5% SDS PAGE; antibody diluted at 1:1000
Western Blot: SSRP1 Antibody [NBP1-33235] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SSRP1 antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, RM, XpSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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SSRP1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SSRP1. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Mouse (100%), Rat (100%), Chicken (99%), Xenopus (91%), Rhesus Macaque (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SSRP1
Purity
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation
  • Western Blot 1:500-1:3000
Application Notes
IP-Assay dependent
Theoretical MW
81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (91%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for SSRP1 Antibody

  • Chromatin-specific transcription elongation factor 80 kDa subunit
  • cisplatin-DNA SSRP
  • facilitates chromatin remodeling 80 kDa subunit
  • Facilitates chromatin transcription complex 80 kDa subunit
  • Facilitates chromatin transcription complex subunit SSRP1
  • FACT complex subunit SSRP1
  • FACT
  • FACT80FACT 80 kDa subunit
  • FACTp80
  • high mobility group box
  • hSSRP1
  • Recombination signal sequence recognition protein 1
  • structure specific recognition protein 1
  • Structure-specific recognition protein 1
  • T160

Background

SSRP1 is a nuclear protein and a component of the FACT complex (facilitates chromatin transcription). The FACT complex is composed of the SUPT16H (FACT140) and the SSRP1 FACTp80 proteins. This complex interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation. This protein interacts with dsDNA. The SSRP1 protein has been shown to be modified by cisplatin and may protect DNA from repair and block DNA replication. The 10D7 monoclonal antibody recognizes the human SSRP1 protein and has been shown to be useful for Western blotting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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SSRP1 Antibody
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Applications: WB, IHC, IP
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Bioinformatics

Gene Symbol SSRP1