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SSEA-1 Antibody (MEM-158) [FITC]

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Flow Cytometry: CD15/Lewis X Antibody (MEM-158) [FITC] [NB500-377] - Fig. 1. Surface staining of human peripheral blood cells with anti-human CD15 (MEM-158) FITC. Cells in the granulocyte gate were used for analysis.
Flow Cytometry: CD15/Lewis X Antibody (MEM-158) [FITC] [NB500-377] - Fig. 1. Surface staining of human peripheral blood cells with anti-human CD15 (MEM-158) FITC. Cells in the granulocyte gate were used for analysis.

Product Details

Summary
Reactivity Hu, Bv(-), Po(-), Sh(-)Species Glossary
Applications Flow, IP
Clone
MEM-158
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
NB110-81634
Unconjugated
NB500-376
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SSEA-1 Antibody (MEM-158) [FITC] Summary

Immunogen
Human granulocytes
Localization
Cell Membrane
Specificity
The antibody MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells. HLDA VI; WS Code AS A053
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
FUT4
Purity
Size Exclusion Chromatography
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Applications/Dilutions

Dilutions
  • Flow Cytometry 20 ul/100 ul of whole blood or 10^6 cells in suspension
  • Immunoprecipitation 1:10-1:500

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
TBS and 0.2% (w/v) high grade BSA (protease free)
Preservative
0.9% Sodium Azide
Purity
Size Exclusion Chromatography

Alternate Names for SSEA-1 Antibody (MEM-158) [FITC]

  • alpha-(1,3)-fucosyltransferase
  • CD15
  • EC 2.4.1
  • EC 2.4.1.-
  • EC 2.4.1.65
  • ELAM-1 ligand fucosyltransferase
  • ELFT
  • FCT3AELAM ligand fucosyltransferase
  • fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
  • Fucosyltransferase 4
  • Fucosyltransferase IV
  • FUC-TIV
  • fucT-IV
  • FUTIV
  • Galactoside 3-L-fucosyltransferase
  • Lewis X
  • LeX
  • SSEA1
  • SSEA-1
  • stage-specific embryonic antigen 1

Background

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol FUT4