![Immunocytochemistry/Immunofluorescence: SSEA-1 Antibody (MC-480) - Azide and BSA Free [NBP2-80961] - SSEA1 antibody was tested in Ntera-2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/SSEA-1-Antibody-MC-480-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80961-img0002.jpg "Immunocytochemistry/Immunofluorescence: SSEA-1 Antibody (MC-480) - Azide and BSA Free [NBP2-80961] - SSEA1 antibody was tested in Ntera-2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red). Image from the standard format of this antibody.")
| Reactivity | Hu, Mu, Rt, PmSpecies Glossary |
| Applications | WB, Simple Western, Flow, ICC/IF, IHC |
| Clone | MC-480 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | F9 tetracarcinoma stem cells (X-irradiated). |
| Isotype | IgM |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | FUT4 |
| Purity | IgM purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This SSEA1 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen and Paraffin. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Tris-Glycine, 0.15 M NaCl |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | IgM purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for SSEA-1 Antibody (NBP2-80961)Find related products by research area.
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| Gene Symbol | FUT4 |
![Flow Cytometry: SSEA-1 Antibody (MC-480) - Azide and BSA Free [NBP2-80961] - An intracellular stain was performed on Jurkat cells with SSEA-1 Antibody (MC-480)NB100-1831 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse IgM Alexa Fluor 488-conjugated secondary antibody. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/SSEA-1-Antibody-MC-480-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80961-img0003.jpg "Flow Cytometry: SSEA-1 Antibody (MC-480) - Azide and BSA Free [NBP2-80961] - An intracellular stain was performed on Jurkat cells with SSEA-1 Antibody (MC-480)NB100-1831 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse IgM Alexa Fluor 488-conjugated secondary antibody. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: SSEA-1 Antibody (MC-480) - Azide and BSA Free [NBP2-80961] - SSEA1 antibody (0.1ug/ml) was tested in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/SSEA-1-Antibody-MC-480-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80961-img0001.jpg "Immunocytochemistry/Immunofluorescence: SSEA-1 Antibody (MC-480) - Azide and BSA Free [NBP2-80961] - SSEA1 antibody (0.1ug/ml) was tested in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x. Image from the standard format of this antibody.")
![Simple Western: SSEA-1 Antibody (MC-480) - Azide and BSA Free [NBP2-80961] - Simple Western lane view shows a specific band for SSEA1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/SSEA-1-Antibody-MC-480-Azide-and-BSA-Free-Simple-Western-NBP2-80961-img0004.jpg "Simple Western: SSEA-1 Antibody (MC-480) - Azide and BSA Free [NBP2-80961] - Simple Western lane view shows a specific band for SSEA1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.")
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