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SSEA-1 Antibody (FUT4/815) - Azide and BSA Free

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications IHC
Clone
FUT4/815
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

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SSEA-1 Antibody (FUT4/815) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-45307).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Purified human SSEA-1 (Uniprot: P22083)
Localization
Cell surface and granular paranuclear
Marker
Reed-Sternberg Cell Marker
Isotype
IgM Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FUT4
Purity
Protein L or PEG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein L or PEG purified

Alternate Names for SSEA-1 Antibody (FUT4/815) - Azide and BSA Free

  • alpha-(1,3)-fucosyltransferase
  • CD15
  • EC 2.4.1
  • EC 2.4.1.-
  • EC 2.4.1.65
  • ELAM-1 ligand fucosyltransferase
  • ELFT
  • FCT3AELAM ligand fucosyltransferase
  • fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
  • Fucosyltransferase 4
  • Fucosyltransferase IV
  • FUC-TIV
  • fucT-IV
  • FUTIV
  • Galactoside 3-L-fucosyltransferase
  • Lewis X
  • LeX
  • SSEA1
  • SSEA-1
  • stage-specific embryonic antigen 1

Background

SSEA1 (stage-specific mouse embryonic antigen) is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis. SSEA-1 is also known as CD15, although SSEA1 is more commonly used in a stem cell research context because SSEA-1 refers to the marker identified specifically by monoclonal antibody, clone MC-480. Clone MC-480 is now the gold standard reagent for mouse embryonic stem cells. Only mouse ESCs express SSEA1; human SSEA1 only stains cells after differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol FUT4