SSEA-1 Antibody (FUT4/815) - IHC-Prediluted Summary
Description |
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining. |
Immunogen |
Purified human SSEA-1 (Uniprot: P22083) |
Localization |
Cell surface and granular paranuclear |
Marker |
Reed-Sternberg Cell Marker |
Isotype |
IgM Kappa |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
FUT4 |
Purity |
Protein L or PEG purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunohistochemistry
- Immunohistochemistry-Paraffin
|
Application Notes |
Hu-chromosome location: 11q21 Molecular weight of antigen: ~220kDa (Glycoprotein) Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application. |
Theoretical MW |
220 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at 4C. |
Buffer |
10 mM PBS with 0.05% BSA |
Preservative |
0.05% Sodium Azide |
Purity |
Protein L or PEG purified |
Alternate Names for SSEA-1 Antibody (FUT4/815) - IHC-Prediluted
Background
SSEA1 (stage-specific mouse embryonic antigen) is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis. SSEA-1 is also known as CD15, although SSEA1 is more commonly used in a stem cell research context because SSEA-1 refers to the marker identified specifically by monoclonal antibody, clone MC-480. Clone MC-480 is now the gold standard reagent for mouse embryonic stem cells. Only mouse ESCs express SSEA1; human SSEA1 only stains cells after differentiation.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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FAQs for SSEA-1 Antibody (NBP2-48370) (0)
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