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SRGAP2 Antibody

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Immunocytochemistry/ Immunofluorescence: SRGAP2 Antibody [NBP1-89013] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: SRGAP2 Antibody [NBP1-89013] - Staining of human Kidney shows moderate nuclear and cytoplasmic positivity in cells in distal tubules.
Immunohistochemistry-Paraffin: SRGAP2 Antibody [NBP1-89013] - Staining of human cerebral cortex shows nuclear positivity.
Immunohistochemistry-Paraffin: SRGAP2 Antibody [NBP1-89013] - Staining of human Bone marrow shows moderate nuclear and cytoplasmic positivity in hematopoietic cells.
Immunohistochemistry-Paraffin: SRGAP2 Antibody [NBP1-89013] - Staining of human Cerebral cortex shows moderate nuclear positivity in glial cells.
Immunohistochemistry-Paraffin: SRGAP2 Antibody [NBP1-89013] - Staining of human Testis shows moderate nuclear and cytoplasmic positivity in Leydig cells and cells in seminiferous ducts.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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SRGAP2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LEPLKTSPVVAPTSEPSSPLHTQLLKDPEPAFQRSASTAGDIACAFRPVKSVKMAAPVKPPATRPKPTVFPKTNATSPGVNSSTSPQST
Predicted Species
Mouse (98%), Rat (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SRGAP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:10-1:20
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
SRGAP2 Protein (NBP1-89013PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SRGAP2 Antibody

  • FNBP2
  • Formin-binding protein 2
  • KIAA0456ARHGAP34formin binding protein 2
  • Rho GTPase-activating protein 34
  • SLIT-ROBO Rho GTPase activating protein 2
  • SLIT-ROBO Rho GTPase-activating protein 2
  • srGAP2
  • SRGAP3

Background

SRGAP2, also known as SLIT-ROBO Rho GTPase-activating protein 2, is a 121 kDa 1071 amino acid protein. Throughout the development of the cerebral cortex SRGAP2 has a significant role in neuronal morphogenesis. Current research on SRGAP2 is being conducted in relation to neuronitis, pilocytic astrocytoma, infantile epileptic encephalopathy and schizophrenia. SRGAP2 is linked to the long-term memory, axon guidance, localization, brain development and cell migration pathways where it interacts with FASLG, YWHAG, YWHAZ, DISC1 and MYO1G.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SRGAP2