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SRGAP1 Antibody (5D2)

Images

 
Western Blot: SRGAP1 Antibody (5D2) [H00057522-M03] - SRGAP1 expression in NIH/3T3 ( Cat # L018V1 ).
Immunohistochemistry-Paraffin: SRGAP1 Antibody (5D2) [H00057522-M03] - Analysis of monoclonal antibody to SRGAP1 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml
Western Blot: SRGAP1 Antibody (5D2) [H00057522-M03] - SRGAP1 expression in IMR-32 ( Cat # L008V1 ).
ELISA: SRGAP1 Antibody (5D2) [H00057522-M03] - Detection limit for recombinant GST tagged SRGAP1 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Rt, PmSpecies Glossary
Applications WB, ELISA, IHC
Clone
5D2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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SRGAP1 Antibody (5D2) Summary

Immunogen
SRGAP1 (NP_065813, 952 a.a. ~ 1050 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PLDPETIAQDIEETMNTALNELRELERQSTAKHAPDVVLDTLEQVKNSPTPATSTESLSPLHNVALRSSEPQIRRSTSSSSDTMSTFKPMVAPRMGVQL
Specificity
SRGAP1 - SLIT-ROBO Rho GTPase activating protein 1
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SRGAP1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Publications
Read Publications using H00057522-M03.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SRGAP1 Antibody (5D2)

  • ARHGAP13srGAP1
  • FLJ22166
  • KIAA1304SLIT-ROBO Rho GTPase-activating protein 1
  • Rho GTPase-activating protein 13
  • SLIT-ROBO Rho GTPase activating protein 1

Background

GTPase-activating protein for RhoA and Cdc42 small GTPases. Together with CDC42 seems to be involved in thepathway mediating the repulsive signaling of Robo and Slit proteins in neuronal migration. SLIT2, probably throughinteraction with ROBO1, increases the interaction of SRGAP1 with ROBO1 and inactivates CDC42

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SRGAP1 Antibody (H00057522-M03)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SRGAP1
Entrez
OMIM
Uniprot