![Western Blot: SPG7 Antibody (OTI1C1) - Azide and BSA Free [NBP2-74327] - Human tissue.](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1C1-Azide-and-BSA-Free-Western-Blot-NBP2-74327-img0003.jpg "Western Blot: SPG7 Antibody (OTI1C1) - Azide and BSA Free [NBP2-74327] - Human tissue.")
| Reactivity | Hu, Mu, Rt, Ca, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, CyTOF-ready |
| Clone | OTI1C1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 300-573 of human SPG7(NP_003110) produced in E.coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SPG7 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 88.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
Secondary Antibodies |
Isotype Controls |
Research Areas for SPG7 Antibody (NBP2-74327)Find related products by research area.
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| Gene Symbol | SPG7 |
![Immunocytochemistry/Immunofluorescence: SPG7 Antibody (OTI1C1) - Azide and BSA Free [NBP2-74327] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SPG7.](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1C1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74327-img0007.jpg "Immunocytochemistry/Immunofluorescence: SPG7 Antibody (OTI1C1) - Azide and BSA Free [NBP2-74327] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SPG7.")
![Flow Cytometry: SPG7 Antibody (OTI1C1) - Azide and BSA Free [NBP2-74327] - Analysis of Jurkat cells, using anti-SPG7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1C1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74327-img0005.jpg "Flow Cytometry: SPG7 Antibody (OTI1C1) - Azide and BSA Free [NBP2-74327] - Analysis of Jurkat cells, using anti-SPG7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Western Blot: SPG7 Antibody (OTI1C1) - Azide and BSA Free [NBP2-74327] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SPG7 monoclonal antibody.](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1C1-Azide-and-BSA-Free-Western-Blot-NBP2-74327-img0001.jpg "Western Blot: SPG7 Antibody (OTI1C1) - Azide and BSA Free [NBP2-74327] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SPG7 monoclonal antibody.")
![Western Blot: SPG7 Antibody (OTI1C1) - Azide and BSA Free [NBP2-74327] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPG7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPG7.](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1C1-Azide-and-BSA-Free-Western-Blot-NBP2-74327-img0002.jpg "Western Blot: SPG7 Antibody (OTI1C1) - Azide and BSA Free [NBP2-74327] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPG7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPG7.")
![Immunocytochemistry/Immunofluorescence: SPG7 Antibody (OTI1C1) - Azide and BSA Free [NBP2-74327] - Staining of HeLa cells using anti-SPG7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1C1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74327-img0006.jpg "Immunocytochemistry/Immunofluorescence: SPG7 Antibody (OTI1C1) - Azide and BSA Free [NBP2-74327] - Staining of HeLa cells using anti-SPG7 mouse monoclonal antibody.")
![Flow Cytometry: SPG7 Antibody (OTI1C1) - Azide and BSA Free [NBP2-74327] - Analysis of Hela cells, using anti-SPG7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1C1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74327-img0004.jpg "Flow Cytometry: SPG7 Antibody (OTI1C1) - Azide and BSA Free [NBP2-74327] - Analysis of Hela cells, using anti-SPG7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")