![Western Blot: SP17 Antibody [NBP1-85409] - Analysis in control (vector only transfected HEK293T lysate) and SPA17 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/SP17-Antibody-Western-Blot-NBP1-85409-img0013.jpg "Western Blot: SP17 Antibody [NBP1-85409] - Analysis in control (vector only transfected HEK293T lysate) and SPA17 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: EQPDNIPAFAAAYFESLLEKREKTNFDPAEWGSKVEDRFYNNHAFEEQEPPEKSDPKQEESQISGKEEETSVTILDSSEEDKEKEEVAAVK |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SPA17 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for SP17 Antibody (NBP1-85409)Find related products by research area.
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| Gene Symbol | SPA17 |
![Immunocytochemistry/Immunofluorescence: SP17 Antibody [NBP1-85409] - Staining of human cell line U-2 OS shows localization to vesicles.](http://images.novusbio.com/fullsize/SP17-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85409-img0012.jpg "Immunocytochemistry/Immunofluorescence: SP17 Antibody [NBP1-85409] - Staining of human cell line U-2 OS shows localization to vesicles.")
![Immunohistochemistry-Paraffin: SP17 Antibody [NBP1-85409] - Staining in human testis and endometrium tissues using anti-SPA17 antibody. Corresponding SPA17 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SP17-Antibody-Immunohistochemistry-Paraffin-NBP1-85409-img0011.jpg "Immunohistochemistry-Paraffin: SP17 Antibody [NBP1-85409] - Staining in human testis and endometrium tissues using anti-SPA17 antibody. Corresponding SPA17 RNA-seq data are presented for the same tissues.")
![Western Blot: SP17 Antibody [NBP1-85409] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/SP17-Antibody-Western-Blot-NBP1-85409-img0005.jpg "Western Blot: SP17 Antibody [NBP1-85409] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: SP17 Antibody [NBP1-85409] - Staining of human endometrium shows low expression as expected.](http://images.novusbio.com/fullsize/SP17-Antibody-Immunohistochemistry-Paraffin-NBP1-85409-img0009.jpg "Immunohistochemistry-Paraffin: SP17 Antibody [NBP1-85409] - Staining of human endometrium shows low expression as expected.")
![Immunohistochemistry-Paraffin: SP17 Antibody [NBP1-85409] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/SP17-Antibody-Immunohistochemistry-Paraffin-NBP1-85409-img0010.jpg "Immunohistochemistry-Paraffin: SP17 Antibody [NBP1-85409] - Staining of human testis shows high expression.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")