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SOX10 Antibody (20B7) [Unconjugated]

 (21 Publications)
 (1 Review)
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SOX10 was detected in immersion fixed BG01V human embryonic stem cells differentiated to neural crest stem cells using Mouse Anti-Human/Rat SOX10 Monoclonal Antibody (Catalog # MAB2864) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
SOX10 was detected in immersion fixed BG01V human embryonic stem cells differentiated to neural crest stem cells using Mouse Anti-Human/Rat SOX10 Monoclonal Antibody (Catalog # MAB2864) at 10 µg/mL for 3 hours at room ...read more
SOX10 was detected in immersion fixed paraffin-embedded sections of human melanoma tissue using Mouse Anti-Human/Rat SOX10 Monoclonal Antibody (Catalog # MAB2864) at 5 µg/mL for 1 hour at room temperature ...read more
Identification of neural crest-derived DPCs. ((a)–(c)) Immunofluorescent cytochemical staining revealed expression of NCSCs-specific markers in DPCs. Primary DPCs were double positive for P75 and Sox10 (a), P75 and ...read more
JCV T-antigen is expressed in neural crest cells.Fluorescent images of bone marrow cells isolated from JCV T-antigen transgenic mice cultured in neural conditions show that these cells are positive for p75 (A,B), nestin ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications IHC, ICC/IF
Clone
20B7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
NL557
NL2864R
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
NL2864R
Carrier Free
MAB2864-SP
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SOX10 Antibody (20B7) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant rat SOX10
aa 1-118
Specificity
Detects human and rat SOX10. Epitope-mapping experiments indicated that clone 20B7 recognizes a determinant in the first 65 amino acids, which contains sequences unique to SOX10 (1).
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SOX10
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB2864 in the following applications:
Publications
Read Publications using
MAB2864 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX10 Antibody (20B7) [Unconjugated]

  • DOM
  • MGC15649
  • PCWH
  • SOX10
  • SRY (sex determining region Y)-box 10
  • SRY-related HMG-box gene 10
  • transcription factor SOX-10
  • WS4
  • WS4C
  • WS4mouse, human homolog of

Background

SOX10 belongs to the SOX family of transcription factors which display diverse roles during development. In the central nervous system, SOX10 is required for the terminal differentiation of oligodendrocytes and myelination. In the peripheral nervous system, SOX10 maintains pluripotency of neural crest stem cells and suppresses neuronal differentiation.
  1. Lo, L. et al. (2002) Development 129:1553.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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SOX10 Antibody
(20B7) [Unconjugated]
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SOX10 Antibody (20B7) [Unconjugated]
Species: Hu, Rt
Applications: IHC, ICC/IF

Publications for SOX10 Antibody (MAB2864)(21)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC, IHC, Immunoprecipitation, Western Blot.


Filter By Application
ICC
(7)
IHC
(2)
Immunoprecipitation
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(4)
Mouse
(1)
Rat
(6)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using MAB2864 Applications Species
Guillemot-Legris, O;Girmahun, G;Shipley, RJ;Phillips, JB; Local Administration of Minocycline Improves Nerve Regeneration in Two Rat Nerve Injury Models International journal of molecular sciences 2023-07-28 [PMID: 37569473] (IHC, Rat) IHC Rat
Dou Ye, Suqing Qu, Yinxiang Yang, Zhaoyan Wang, Qian Wang, Weipeng Liu et al. Intrauterine desensitization enables long term survival of human oligodendrocyte progenitor cells without immunosuppression iScience 2023-05-19 [PMID: 37168574]
Ye D, Wang Q, Yang Y et al. Identifying Genes that Affect Differentiation of Human Neural Stem Cells and Myelination of Mature Oligodendrocytes Cellular and molecular neurobiology 2022-12-22 [PMID: 36547781]
Robin Reschke, Jovian Yu, Blake A Flood, Emily F Higgs, Ken Hatogai, Thomas F Gajewski Immune cell and tumor cell-derived CXCL10 is indicative of immunotherapy response in metastatic melanoma Journal for ImmunoTherapy of Cancer 2021-09-29 [PMID: 34593622]
C Laberthonn, EM Novoa-Del-, R Chevalier, N Broucqsaul, VV Rao, JP Trani, K Nguyen, S Xue, B Reversade, JD Robin, A Baudot, F Magdinier AKT Signaling Modifies the Balance between Cell Proliferation and Migration in Neural Crest Cells from Patients Affected with Bosma Arhinia and Microphthalmia Syndrome Biomedicines, 2021-06-29;9(7):. 2021-06-29 [PMID: 34209568] (IHC, Human) IHC Human
Alexander Xi Fu, Kathy Nga-Chu Lui, Clara Sze-Man Tang, Ray Kit Ng, Frank Pui-Ling Lai, Sin-Ting Lau et al. Whole-genome analysis of noncoding genetic variations identifies multiscale regulatory element perturbations associated with Hirschsprung disease Genome Research 2020-11-01 [PMID: 32948616]
RC Ching, M Wiberg, PJ Kingham Schwann cell-like differentiated adipose stem cells promote neurite outgrowth via secreted exosomes and RNA transfer Stem Cell Res Ther, 2018-10-11;9(1):266. 2018-10-11 [PMID: 30309388] (ICC, Rat) ICC Rat
Philip T. T. Ly, Craig Stewart, Catherine J. Pallen PTP alpha is required for laminin-2-induced Fyn-Akt signaling to drive oligodendrocyte differentiation Journal of Cell Science 2018-01-01 [PMID: 29991512]
Janardhan KS, Jensen H, Clayton NP, Herbert RA Immunohistochemistry in Investigative and Toxicologic Pathology Toxicol Pathol. 7/1/2018 [PMID: 29966501]

Details:
Citation used the HRP format of this antibody.
JC Cronin, SK Loftus, LL Baxter, S Swatkoski, M Gucek, WJ Pavan Identification and functional analysis of SOX10 phosphorylation sites in melanoma PLoS ONE, 2018-01-09;13(1):e0190834. 2018-01-09 [PMID: 29315345] (Immunoprecipitation, Human) Immunoprecipitation Human
Show All 21 Publications.

Review for SOX10 Antibody (MAB2864) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB2864:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
 IHC-P Human 12/05/2022
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedhuman melanoma
SpeciesHuman
LotWZL0221111

FAQs for SOX10 Antibody (MAB2864) (0)

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Immunohistochemistry-Paraffin SOX10 MAB2864
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 Verified Customer
12/05/2022
Application: IHC-P
Species: Human
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Bioinformatics

Gene Symbol SOX10