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SMC3 Antibody

Images

 
Western Blot: SMC3 Antibody [NBP1-88715] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: U-251 MG
Immunohistochemistry: SMC3 Antibody [NBP1-88715] - Staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.
Simple Western: SMC3 Antibody [NBP1-88715] - Simple Western lane view shows a specific band for SMC3 in 0.2 mg/ml of U-251 MG lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Simple Western: SMC3 Antibody [NBP1-88715] - Electropherogram image of the corresponding Simple Western lane view. SMC3 antibody was used at 1:25 dilution on U-251 MG lysate(s) respectively.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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SMC3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EFNKMNLPGEVTFLPLNKLDVRDTAYPETNDAIPMISKLRYNPRFDKAFKHVFGKTLICRSMEVSTQLARAFTMDCITLEGDQVSHRG
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMC3
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Simple Western 1:25
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Control Peptide
SMC3 Protein (NBP1-88715PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SMC3 Antibody

  • Bamacan
  • BAMSMC protein 3
  • Basement membrane-associated chondroitin proteoglycan
  • BMH
  • chondroitin sulfate proteoglycan 6 (bamacan)
  • Chondroitin sulfate proteoglycan 6
  • Chromosome-associated polypeptide
  • CSPG6structural maintenance of chromosomes protein 3
  • hCAP
  • HCAPbamacan
  • SMC-3
  • SMC3L1CDLS3
  • structural maintenance of chromosomes 3

Background

Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis and in chromosome movement.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SMC3