![Western Blot: SLC39A7/ZIP7 Antibody [NBP1-76504] - Western Blot Validation in Rat Brain Tissue Lysate. Loading: 15 ug of lysates per lane. Antibodies: ZIP7, NBP1-76504 (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/SLC39A7-ZIP7-Antibody-Western-Blot-NBP1-76504-img0008.jpg "Western Blot: SLC39A7/ZIP7 Antibody [NBP1-76504] - Western Blot Validation in Rat Brain Tissue Lysate. Loading: 15 ug of lysates per lane. Antibodies: ZIP7, NBP1-76504 (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human SLC39A7/ZIP7. The immunogen is located within amino acids 20-70 of SLC39A7/ZIP7. Amino Acid Sequence: HDDLQEDFHGHSHRHSH |
| Specificity | Human SLC39A7/ZIP7 has one isoform (469aa, 50kD). Mouse SLC39A7/ZIP7 has one isoform (476aa, 51kD) and Rat SLC39A7/ZIP7 also has one isoform (468aa, 50kD). NBP1-76504 can detect human, mouse and rat. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SLC39A7 |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
![Immunohistochemistry ZnT-8/SLC30A8 Antibody (815039) [Unconjugated]](https://images.novusbio.com/images2/ZnT8_MAB7936_Immunohistochemistry_12988.jpg)
![Intracellular Staining by Flow Cytometry ZnT-8/SLC30A8 Antibody (815039) [Unconjugated]](https://images.novusbio.com/images2/ZnT8_MAB7936_Flow_Cytometry_23901.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for SLC39A7/ZIP7 Antibody (NBP1-76504)Find related products by research area.
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| Gene Symbol | SLC39A7 |
| Uniprot |
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![Immunocytochemistry/Immunofluorescence: SLC39A7/ZIP7 Antibody [NBP1-76504] - Immunofluorescence of ZIP7 in human brain tissue with ZIP7 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/SLC39A7-ZIP7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76504-img0002.jpg "Immunocytochemistry/Immunofluorescence: SLC39A7/ZIP7 Antibody [NBP1-76504] - Immunofluorescence of ZIP7 in human brain tissue with ZIP7 antibody at 20 ug/mL.")
![Western Blot: SLC39A7/ZIP7 Antibody [NBP1-76504] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: ZIP7, NBP1-76504 (1 ug/mL), ZIP7 from Company B (4 ug/mL), and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/SLC39A7-ZIP7-Antibody-Western-Blot-NBP1-76504-img0004.jpg "Western Blot: SLC39A7/ZIP7 Antibody [NBP1-76504] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: ZIP7, NBP1-76504 (1 ug/mL), ZIP7 from Company B (4 ug/mL), and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: SLC39A7/ZIP7 Antibody [NBP1-76504] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: ZIP7, NBP1-76504 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/SLC39A7-ZIP7-Antibody-Western-Blot-NBP1-76504-img0005.jpg "Western Blot: SLC39A7/ZIP7 Antibody [NBP1-76504] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: ZIP7, NBP1-76504 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: SLC39A7/ZIP7 Antibody [NBP1-76504] - Western Blot Validation in Human Brain Tissue and 3T3 (Balb) Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: ZIP7, NBP1-76504 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/SLC39A7-ZIP7-Antibody-Western-Blot-NBP1-76504-img0006.jpg "Western Blot: SLC39A7/ZIP7 Antibody [NBP1-76504] - Western Blot Validation in Human Brain Tissue and 3T3 (Balb) Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: ZIP7, NBP1-76504 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: SLC39A7/ZIP7 Antibody [NBP1-76504] - Western Blot Validation in Mouse Brain Tissue Lysate. Loading: 15 ug of lysates per lane. Antibodies: ZIP7, NBP1-76504 (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/SLC39A7-ZIP7-Antibody-Western-Blot-NBP1-76504-img0007.jpg "Western Blot: SLC39A7/ZIP7 Antibody [NBP1-76504] - Western Blot Validation in Mouse Brain Tissue Lysate. Loading: 15 ug of lysates per lane. Antibodies: ZIP7, NBP1-76504 (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunohistochemistry: SLC39A7/ZIP7 Antibody - BSA Free [NBP1-76504] - Figure 6 Immunohistochemistry Validation of SLC39A7/ZIP7 in Human Brain Tissue Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-SLC39A7/ZIP7 antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76504_rabbit-polyclonal-slc39a7-zip7-antibody-2711202420105817.jpg "Immunohistochemistry: SLC39A7/ZIP7 Antibody - BSA Free [NBP1-76504] - Figure 6 Immunohistochemistry Validation of SLC39A7/ZIP7 in Human Brain Tissue Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-SLC39A7/ZIP7 antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![SDS-PAGE ErbB2/Her2 [Unconjugated]](https://images.novusbio.com/images2/ErbB2_1129-ER_70.jpg)
![Bioactivity ErbB2/Her2 [Unconjugated]](https://images.novusbio.com/images2/1129-er_recombinant-human-erbb2-her2-fc-chimera-protein-cf-bioactivity-7122020142841.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")