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Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) - Azide and BSA Free

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Protein Array: Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) - Azide and BSA Free [NBP3-14290] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11).
Protein Array: Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) - Azide and BSA Free [NBP3-14290] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Sirtuin 1/SIRT1 Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IP, MA
Clone
PCRP-SIRT1-1E11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-14290AF350
Alexa Fluor 405
NBP3-14290AF405
Alexa Fluor 488
NBP3-14290AF488
Alexa Fluor 532
NBP3-14290AF532
Alexa Fluor 594
NBP3-14290AF594
Alexa Fluor 647
NBP3-14290AF647
Alexa Fluor 700
NBP3-14290AF700
Alexa Fluor 750
NBP3-14290AF750
Allophycocyanin
NBP3-14290APC
Biotin
NBP3-14290B
CoraFluor 1
NBP3-14290CL1
DyLight 350
NBP3-14290UV
DyLight 405
NBP3-14290V
DyLight 488
NBP3-14290G
DyLight 550
NBP3-14290R
DyLight 594
NBP3-14290DL594
DyLight 650
NBP3-14290C
DyLight 680
NBP3-14290FR
DyLight 755
NBP3-14290IR
FITC
NBP3-14290F
HRP
NBP3-14290H
Janelia Fluor 525
NBP3-14290JF525
Janelia Fluor 549
NBP3-14290JF549
Janelia Fluor 646
NBP3-14290JF646
Janelia Fluor 669
NBP3-14290JF669
PE
NBP3-14290PE
PerCP
NBP3-14290PCP
Unconjugated
NBP3-13916-100ug
mFluor Violet 450 SE
NBP3-14290MFV450
mFluor Violet 500 SE
NBP3-14290MFV500
mFluor Violet 610 SE
NBP3-14290MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-13916-100ug
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Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13916).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Immunogen
Recombinant full-length human Sirtuin 1/SIRT1protein (Uniprot: Q96EB6)
Localization
Cytoplasm, Mitochondrion and Nucleus, PML body
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SIRT1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunoprecipitation 1-2 ug per 100-500 ug protein
  • Protein Array
  • Western Blot 1-2 ug/ml
Application Notes
ELISA: For coating, order this antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) - Azide and BSA Free

  • EC 3.5.1
  • S. cerevisiae, homolog) 1
  • SIR2alpha
  • SIR2L1
  • SIR2-like protein 1
  • SIRT1
  • Sirtuin 1

Background

The S. cerevisiae silent information regulator 2 (Sir2) protein is an NAD+-dependent histone deacetylase (HDACs) that plays a critical role in transcriptional silencing, genome stability, and longevity (1-2). Part of the Sirtuin family (Sir2, Sir3, Sir3), yeast Sir2 is the founding member of Class III HDACs. A human homologue of Sir2 , SIRT1, regulates the activity of the p53 tumor suppressor and inhibits apoptosis (3-4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SIRT1