SIRT6 Antibody

SIRT6 (SIR2-like protein 6) is a member of the yeast silent information regulator 2 (SIR2) family and possesses both strong ADP-ribosyltransferase activity as well as protein deacetylase activity which is dependent on NAD(+). SIRT6 exerts deacetylase activity towards histone H3K9Ac and H3K56Ac, and alters histone H3 acetylation in telomeric chromatin during cell cycle's S-phase. It deacetylates histone H3K9Ac at NFkB target promoters and downregulates a subset of NFkB target genes. Upon DNA damage, SIRT6 promotes DNA end resection via deacetylation of RBBP8. SIRT6 also serves as a corepressor of the transcription factor HIF1 alpha for regulating the expression of multiple glycolytic genes to control glucose homeostasis, and is required for normal IGF1 serum levels. SIRT6 play critical roles in maintaining telomere integrity, fine-tuning aging-associated gene expression programs, preventing genomic instability, and maintaining metabolic homeostasis, thus impacting on several pathways relevant for cancer, metabolism, aging, cellular senescence and apoptosis.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 8 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-P, IP, KD, WB, Western Blot.

Showing Publications 1 - 10 of 20. Show All 20 Publications. Collapse Publications.

Publications using NB100-2522	Applications	Species
Zhu Y, Hu S, Pan X et al. Hepatocyte Sirtuin 6 Protects against Atherosclerosis and Steatohepatitis by Regulating Lipid Homeostasis Cells 2023-08-05 [PMID: 37566087] (Western Blot, Mouse)	Western Blot	Mouse
Kurabayashi A, Iwashita W, Tanaka C et al. Murine remote ischemic preconditioning suppresses diabetic ketoacidosis by enhancing glycolysis and entry into tricarboxylic acid cycle in the liver Life Sci. 2020-05-05 [PMID: 32387415] (WB, Mouse)	WB	Mouse
Zhong, X, Huang, M Et al. SIRT6 Protects Against Liver Fibrosis by Deacetylation and Suppression of SMAD3 in Hepatic Stellate Cells. Cell Mol Gastroenterol Hepatol 2020-04-20 [PMID: 32305562] (IF/IHC, Mouse)	IF/IHC	Mouse
Zhang L, Bai L, Ren Q et al. Protective effects of SIRT6 against lipopolysaccharide (LPS) are mediated by deacetylation of Ku70. Mol. Immunol. 2018-07-19 [PMID: 30032073] (WB, Human)	WB	Human
Xia YQ, Hua RJ, Juan C et al. SIRT6 Depletion Sensitizes Human Hepatoma Cells to Chemotherapeutics by Downregulating MDR1 Expression. Front Pharmacol. 2018-03-06 [PMID: 29563873] (IP, Human)	IP	Human
Wang, L, Guo, W Et al. Aberrant SIRT6 expression contributes to melanoma growth: Role of the autophagy paradox and IGF-AKT signaling. Autophagy 2017-12-08 [PMID: 29215322] (WB, Human)	WB	Human
Tao NN, Ren JH, Tang H et al. Deacetylation of Ku70 by SIRT6 attenuates Bax-mediated apoptosis in hepatocellular carcinoma. Biochem. Biophys. Res. Commun. 2017-02-23 [PMID: 28238784] (WB)	WB
Ran LK, Chen Y, Zhang ZZ et al. SIRT6 Overexpression Potentiates Apoptosis Evasion in Hepatocellular Carcinoma via BCL2-Associated X Protein-Dependent Apoptotic Pathway. Clin Cancer Res 2016-07-01 [PMID: 26861461]
Meng F, Qian M, Peng B et al. A synergistic action of sirtuins directly recognizes DNA breaks and potentiates DNA damage response and repair bioRxiv 2020-01-01 (WB, KD, Human)	WB, KD	Human
Zhou HZ, Zeng HQ, Yuan D et al. NQO1 potentiates apoptosis evasion and upregulates XIAP via inhibiting proteasome-mediated degradation SIRT6 in hepatocellular carcinoma Cell Commun. Signal 2019-12-16 [PMID: 31842909] (WB, Human)	WB	Human
Fukuda T, Wada-Hiraike O, Oda K et al. Putative tumor suppression function of SIRT6 in endometrial cancer FEBS Lett. 2015-08-04 [PMID: 26183563] (WB, Human)	WB	Human
Oikawa S, Mano A, Takahashi R, Kakinuma Y. Remote ischemic preconditioning with a specialized protocol activates the non-neuronal cardiac cholinergic system and increases ATP content in the heart Int. Immunopharmacol. 2015-06-11 [PMID: 26072685] (WB, Mouse)	WB	Mouse
Zwaans BMM. EXPLORING THE ROLES OF THE HISTONE DEACETYLASE AND LONGEVITY FACTOR SIRT6 IN CANCER Thesis. 2014-01-01 (WB, IHC-P, Human, Mouse)	WB, IHC-P	Human, Mouse
Orellana ME, Quezada C, Maloney SC et al. Expression of SIRT2 and SIRT6 in Retinoblastoma Ophthalmic Res. 2015-02-09 [PMID: 25660418] (IHC-P, Human)	IHC-P	Human
Cardinale A, De Stefano Mc, Mollinari C et al. Biochemical Characterization of Sirtuin 6 in the Brain and Its Involvement in Oxidative Stress Response Neurochem. Res. 2014-11-01 [PMID: 25366464] (WB, IHC-Fr, ICC/IF, Mouse) Details: SIRT6 antibody used for IHC-Fr on 30uM coronal sectiions of cortical and hippocampal regions of mice (Fig 1). Antibody also used for ICC-IF on primary cortical and hippocampal neurons from E18 mice (Fig 3a). Antibody also used for WB of SIRT6 in cortical and hippocampal regions of mice (Fig 2), on cultured primary cortical / hippocampal neurons from E18 mice (Fig 3B) and on whole protein extracts derived from uninfected, rAAV-HA and rAAV-HA-SIRT6 infected cortical neuron (Fig 4B)	WB, IHC-Fr, ICC/IF	Mouse
Tran L, Schulkin J, Ligon Co et al. Epigenetic modulation of chronic anxiety and pain by histone deacetylation. Mol. Psychiatry. 2014-10-07 [PMID: 25288139] (ICC/IF, IP, Rat)	ICC/IF, IP	Rat
Thirumurthi Umadevi, Shen Jia, Xia Weiya et al. MDM2-mediated degradation of SIRT6 phosphorylated by AKT1 promotes tumorigenesis and trastuzumab resistance in breast cancer. Sci Signal. 2014-07-29 [PMID: 25074979] (IF/IHC)	IF/IHC
Lee OH, Kim J, Kim JM et al. Decreased expression of sirtuin 6 is associated with release of high mobility group box-1 after cerebral ischemia. Biochem Biophys Res Commun 2013-07-27 [PMID: 23899523] (ICC/IF, Rat)	ICC/IF	Rat
Sundaresan NR, Vasudevan P, Zhong L et al. The sirtuin SIRT6 blocks IGF-Akt signaling and development of cardiac hypertrophy by targeting c-Jun Nat Med 2012-10-21 [PMID: 23086477] (WB, Mouse)	WB	Mouse
Min L, Ji Y, Bakiri L et al. Liver cancer initiation is controlled by AP-1 through SIRT6-dependent inhibition of survivin Nat Cell Biol 2012-10-07 [PMID: 23041974] (IF/IHC, WB, Mouse)	IF/IHC, WB	Mouse
Show All 20 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	WB	Mouse	04/04/2020	View Summary Application Western Blot Sample Tested 4T1 mouse breast cancer cell line Species Mouse Lot C-1
Enlarge	4 reviewed by: Verified Customer	WB	Human	12/27/2019	View Summary Application Western Blot Sample Tested THP-1 cell lysate Species Human Lot C-1
Enlarge	5 reviewed by: Verified Customer	WB	Human	10/17/2018	View Summary Application Western Blot Sample Tested AR42J human acinar pancreatic cancer cell line,Human Panc-1 Cell Line Species Human Lot C-1

Array NB100-2522

• SIRT6 Antibodies
• SIRT6 ELISA Kits
• SIRT6 Lysate
• SIRT6 Proteins