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SIRP alpha/CD172a Antibody - BSA Free

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Western Blot: SIRP alpha/CD172a Antibody [NBP1-77045] - Analysis of SIRP alpha in THP-1 whole cell lysate with anti-SIRP alpha using this antibody at 1:2000 dilution.
Immunocytochemistry/ Immunofluorescence: SIRP alpha/CD172a Antibody [NBP1-77045] - THP-1 cells with SIRP alpha antibody at 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: SIRP alpha/CD172a Antibody - BSA Free [NBP1-77045] - Immunocytochemistry of SIRP alpha in THP-1 cells with SIRP alpha antibody at 1 u/mL.
Immunocytochemistry/ Immunofluorescence: SIRP alpha/CD172a Antibody - BSA Free [NBP1-77045] - Immunocytochemistry of SIRP alpha in THP-1 cells with SIRP alpha antibody at 1 u/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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SIRP alpha/CD172a Antibody - BSA Free Summary

Immunogen
Antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human SIRP alpha 1. The immunogen is located within the last 50 amino acids of SIRP alpha. Amino Acid Squence: KPEPSFSEYASVQVPRK
Specificity
Recognizes SIRP alpha 1, 2 and 3.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SIRPA
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1 ug/ml
  • Western Blot 0.5-1 ug/ml
Application Notes
75 - 110 kDa bands should be detected in Western Blot.
Theoretical MW
75-110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
SIRP alpha/CD172a Peptide (NBP1-77045PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (88%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for SIRP alpha/CD172a Antibody - BSA Free

  • BIT
  • BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs
  • Brain Ig-like molecule with tyrosine-based activation motifs
  • CD172 antigen-like family member A
  • CD172a antigen
  • CD172a
  • Inhibitory receptor SHPS-1
  • Macrophage fusion receptor
  • MFR
  • MFRtyrosine phosphatase SHP substrate 1
  • MyD-1 antigen
  • MYD1
  • MYD-1
  • P84
  • protein tyrosine phosphatase, non-receptor type substrate 1
  • PTPNS1
  • SHP substrate 1
  • SHPS1
  • SHPS-1
  • SHPS1CD172A
  • signal-regulatory protein alpha
  • Signal-regulatory protein alpha-1
  • Signal-regulatory protein alpha-2
  • Signal-regulatory protein alpha-3
  • SIRP alpha
  • SIRPA
  • SIRPalpha
  • Sirp-alpha-1
  • SIRPalpha2
  • Sirp-alpha-2
  • Sirp-alpha-3
  • SIRPtyrosine-protein phosphatase non-receptor type substrate 1

Background

Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPalpha, SHPS-1, MyD-1, BIT and p84. SIRPalpha is a new gene family containing at least fifteen members. SIRPalpha is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2. SIRPalpha has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SIRPA
Uniprot