![Western Blot: Siglec-2/CD22 Antibody (1A3A11) [NBP2-37328] - Western blot analysis using CD22 mAb against HEK293 (1) and CD22 (AA: 621-725)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-1A3A11-Western-Blot-NBP2-37328-img0003.jpg "Western Blot: Siglec-2/CD22 Antibody (1A3A11) [NBP2-37328] - Western blot analysis using CD22 mAb against HEK293 (1) and CD22 (AA: 621-725)-hIgGFc transfected HEK293 (2) cell lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, CyTOF-ready |
| Clone | 1A3A11 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human Siglec-2/CD22 (AA: 621-725) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CD22 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is Cytof ready. |
| Theoretical MW | 95.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Siglec-2/CD22 Antibody (NBP2-37328)Find related products by research area.
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Antigen-loss relapse after successful CAR-T therapy; What do we do now? By Jacqueline Carrico, BS, MD Tumor cell mechanisms driving tolerance to CAR-T Despite very promising results of CAR-T therapy in acute lymphoblastic leukemia, B-ALL, antigen-loss relapse has arisen as a major chall... Read full blog post. |
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| Gene Symbol | CD22 |
![Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (1A3A11) [NBP2-37328] - Immunofluorescence analysis of Hela cells using CD22 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-1A3A11-Immunocytochemistry-Immunofluorescence-NBP2-37328-img0004.jpg "Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (1A3A11) [NBP2-37328] - Immunofluorescence analysis of Hela cells using CD22 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.")
![Flow Cytometry: Siglec-2/CD22 Antibody (1A3A11) [NBP2-37328] - Flow cytometric analysis of Hela cells using CD22 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-1A3A11-Flow-Cytometry-NBP2-37328-img0005.jpg "Flow Cytometry: Siglec-2/CD22 Antibody (1A3A11) [NBP2-37328] - Flow cytometric analysis of Hela cells using CD22 mouse mAb (green) and negative control (red).")
![Western Blot: Siglec-2/CD22 Antibody (1A3A11) [NBP2-37328] - Western blot analysis using CD22 mAb against human CD22 recombinant protein. (Expected MW is 37 kDa)](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-1A3A11-Western-Blot-NBP2-37328-img0002.jpg "Western Blot: Siglec-2/CD22 Antibody (1A3A11) [NBP2-37328] - Western blot analysis using CD22 mAb against human CD22 recombinant protein. (Expected MW is 37 kDa)")
![ELISA: Siglec-2/CD22 Antibody (1A3A11) [NBP2-37328] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-1A3A11-ELISA-NBP2-37328-img0001.jpg "ELISA: Siglec-2/CD22 Antibody (1A3A11) [NBP2-37328] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")