![Immunohistochemistry: Siglec-15 Antibody (3C9C3) [NBP3-27166] - Analysis of paraffin-embedded bladder cancer tissues using Siglec-15 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27166_mouse-siglec-15-mab-3c9c3-bsa-free-10420249181910.jpg%3Fv=1666705394 "Immunohistochemistry: Siglec-15 Antibody (3C9C3) [NBP3-27166] - Analysis of paraffin-embedded bladder cancer tissues using Siglec-15 mouse mAb with DAB staining.")
| Reactivity | Hu, PmSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC |
| Clone | 3C9C3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human Siglec-15 (AA: Extra(20-263)) expressed in Mammal. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SIGLEC15 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 35.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
![Immunohistochemistry-Paraffin DAP10/HCST Antibody (982101) [Unconjugated]](https://images.novusbio.com/images/DAP10-HCST-Antibody-982101-Unconjugated-Immunohistochemistry-Paraffin-MAB97861-img0001.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Siglec-15 Antibody (NBP3-27166)Find related products by research area.
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| Gene Symbol | SIGLEC15 |
![Immunocytochemistry/Immunofluorescence: Siglec-15 Antibody (3C9C3) [NBP3-27166] - Analysis of Hela cells using Siglec-15 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/nbp3-27166_mouse-siglec-15-mab-3c9c3-bsa-free-1042024920215.jpg%3Fv=1666705394 "Immunocytochemistry/Immunofluorescence: Siglec-15 Antibody (3C9C3) [NBP3-27166] - Analysis of Hela cells using Siglec-15 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.")
![ELISA: Siglec-15 Antibody (3C9C3) [NBP3-27166] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27166_mouse-siglec-15-mab-3c9c3-bsa-free-1042024921150.jpg%3Fv=1666705394 "ELISA: Siglec-15 Antibody (3C9C3) [NBP3-27166] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)")
![Western Blot: Siglec-15 Antibody (3C9C3) [NBP3-27166] - Analysis using Siglec-15 mouse mAb against PC-2 (1), LNCap (2), HEK293 (3), PC-3 (4), DU145 (5), COS-7 (6), and HEK293-6e (7) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27166_mouse-siglec-15-mab-3c9c3-bsa-free-1042024916213.jpg%3Fv=1666705394 "Western Blot: Siglec-15 Antibody (3C9C3) [NBP3-27166] - Analysis using Siglec-15 mouse mAb against PC-2 (1), LNCap (2), HEK293 (3), PC-3 (4), DU145 (5), COS-7 (6), and HEK293-6e (7) cell lysate.")
![Flow Cytometry: Siglec-15 Antibody (3C9C3) [NBP3-27166] - Analysis of THP-1 cells using Siglec-15 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27166_mouse-siglec-15-mab-3c9c3-bsa-free-1042024920226.jpg%3Fv=1666705394 "Flow Cytometry: Siglec-15 Antibody (3C9C3) [NBP3-27166] - Analysis of THP-1 cells using Siglec-15 mouse mAb (green) and negative control (red).")
![Western Blot: Siglec-15 Antibody (3C9C3) [NBP3-27166] - Analysis using Siglec-15 mAb against human Siglec-15 (AA: Extra(20-263)) recombinant protein. (Expected MW is 56.4 kDa)](http://images.novusbio.com/fullsize/nbp3-27166_mouse-siglec-15-mab-3c9c3-bsa-free-1042024920214.jpg%3Fv=1666705394 "Western Blot: Siglec-15 Antibody (3C9C3) [NBP3-27166] - Analysis using Siglec-15 mAb against human Siglec-15 (AA: Extra(20-263)) recombinant protein. (Expected MW is 56.4 kDa)")
![Flow Cytometry: Siglec-15 Antibody (3C9C3) [NBP3-27166] - Analysis of Jurkat cells using Siglec-15 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27166_mouse-siglec-15-mab-3c9c3-bsa-free-1042024915820.jpg%3Fv=1666705394 "Flow Cytometry: Siglec-15 Antibody (3C9C3) [NBP3-27166] - Analysis of Jurkat cells using Siglec-15 mouse mAb (green) and negative control (red).")
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![SDS-PAGE TRANCE/TNFSF11/RANK L [Unconjugated]](https://images.novusbio.com/images2/TRANCE_462-TEC_442.jpg)
![Bioactivity TRANCE/TNFSF11/RANK L [Unconjugated]](https://images.novusbio.com/images2/TRANCE_462-TEC_556.jpg)
![N/A CD40 Ligand/TNFSF5 [HRP]](https://images.novusbio.com/images2/CD40_Ligand_DCDL40_ELISA_60.jpg)
![N/A CD40 Ligand/TNFSF5 [HRP]](https://images.novusbio.com/images2/DATA_CD40_Ligand_DCDL40_ELISA_624.jpg)
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![N/A CD14 [HRP]](https://images.novusbio.com/images2/CD14_DC140_ELISA_42.jpg)
![N/A CD14 [HRP]](https://images.novusbio.com/images2/DATA_CD14_DC140_ELISA_597.jpg)
![Bioactivity IL-2 [Unconjugated]](https://images.novusbio.com/images/202-il_recombinant-human-il-2-protein-bioactivity-174202314946.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")