![Western Blot: SHMT2 Antibody [NBP2-20354] - A. 30 ug A431 whole cell lysate/extract B. 30 ug HepG2 whole cell ysate/extract 7.5 % SDS-PAGE SHMT2 antibody dilution: 1:5000](http://images.novusbio.com/fullsize/SHMT2-Antibody-Western-Blot-NBP2-20354-img0006.jpg "Western Blot: SHMT2 Antibody [NBP2-20354] - A. 30 ug A431 whole cell lysate/extract B. 30 ug HepG2 whole cell ysate/extract 7.5 % SDS-PAGE SHMT2 antibody dilution: 1:5000")
| Reactivity | Hu, Mu, Bv, RbSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human SHMT2. The exact sequence is proprietary. |
| Localization | Mitochondrion |
| Predicted Species | Mouse (93%), Rabbit (93%), Bovine (92%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SHMT2 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 56 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
![Western Blot Peroxiredoxin 5 Antibody [Unconjugated]](https://images.novusbio.com/images2/Peroxiredoxin_5_AF5724_Western_Blot_5819.jpg)
![Immunocytochemistry Peroxiredoxin 5 Antibody [Unconjugated]](https://images.novusbio.com/images2/Peroxiredoxin_5_AF5724_Immunocytochemistry__Immunofluorescence_19720.jpg)
![Immunocytochemistry Peroxiredoxin 5 Antibody [Unconjugated]](https://images.novusbio.com/images2/Peroxiredoxin_5_AF5724_Immunocytochemistry__Immunofluorescence_19954.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SHMT2 |
![Immunocytochemistry/Immunofluorescence: SHMT2 Antibody [NBP2-20354] - HeLa cells were fixed in ice-cold MeOH for 1 sec. Green: SHMT2 protein stained by SHMT2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/SHMT2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20354-img0005.jpg "Immunocytochemistry/Immunofluorescence: SHMT2 Antibody [NBP2-20354] - HeLa cells were fixed in ice-cold MeOH for 1 sec. Green: SHMT2 protein stained by SHMT2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP2-20354] - Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/SHMT2-Antibody-Immunohistochemistry-Paraffin-NBP2-20354-img0002.jpg "Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP2-20354] - Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using antibody at 1:500 dilution.")
![Western Blot: SHMT2 Antibody [NBP2-20354] - Sample (whole cell lysate) A: 293T 10ug, B: HeLa 10ug, 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/SHMT2-Antibody-Western-Blot-NBP2-20354-img0001.jpg "Western Blot: SHMT2 Antibody [NBP2-20354] - Sample (whole cell lysate) A: 293T 10ug, B: HeLa 10ug, 7. 5% SDS PAGE gel, diluted at 1:10000.")
![Immunohistochemistry PTHLH/PTHrP Antibody (677939) [Unconjugated]](https://images.novusbio.com/images2/PTHLH_MAB6734_Immunohistochemistry_10816.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")