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SHANK1 Antibody - BSA Free

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Immunohistochemistry: SHANK1 Antibody [NB300-167] - Synaptic distribution of Shank1/2 and somato-synaptic distribution of Shank3 in the mouse hippocampus, codistribution with VGLUT1, no codistribution with VGLUT2. 5x ...read more
Immunohistochemistry: SHANK1 Antibody [NB300-167] - Mouse retina using NB300-167.
Western Blot: SHANK1 Antibody [NB300-167] - Shank1a in rat brain lysate using NB300-167 at 1:500 dilution.
Immunohistochemistry: SHANK1 Antibody [NB300-167] - Synaptic distribution of Shank1/2 and somato-synaptic distribution of Shank3 in the mouse hippocampus, codistribution with VGLUT1, no codistribution with VGLUT2. ...read more
Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody [NB300-167] - An ASD biometal profile affects cell health and synapse numbers in vitro. Immunocytochemistry of hippocampal neurons DIV 14 grown under control, ...read more
Immunohistochemistry: SHANK1 Antibody [NB300-167] - Colocalization of Shank puncta with VGLUT1 puncta in the intragranular mossy fibers and the stratum moleculare of the dentate gyrus. Confocal immunofluorescence ...read more
Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody [NB300-167] - Colocalization of Shank immunoreactivity with VGLUT1 in the stratum radiatum/stratum lucidum of the CA3 region. confocal immunofluorescence ...read more
Immunohistochemistry: SHANK1 Antibody [NB300-167] - Colocalization of Shank puncta with VGLUT1 puncta in the stratum radiatum of the CA1. confocal immunofluorescence stainings of coronal sections from wild-type mice ...read more
Immunohistochemistry: SHANK1 Antibody [NB300-167] - Retinal tissue using NB300-167 at 1:800 dilution. Images are 2048 X 2048, 12 bit, collected with a 40X water objective, 10 stacks 0.55 um per stack.
Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] - Synaptic distribution of Shank1/2 & somato-synaptic distribution of Shank3 in the mouse hippocampus, codistribution with VGLUT1, no ...read more
Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] - Synaptic distribution of Shank1/2 & somato-synaptic distribution of Shank3 in the mouse hippocampus, codistribution with VGLUT1, no ...read more
Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] - No colocalization of Shank puncta with VGLUT2 puncta in the VGLUT2-band nor in the intragranular mossy fibers of the dentate gyrus. Top ...read more
Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] - Colocalization of Shank puncta with VGLUT1 puncta in the stratum radiatum of the CA1. Top box: Schematic overview of hippocampus with ...read more
Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] - Colocalization of Shank puncta with VGLUT1 puncta in the intragranular mossy fibers & the stratum moleculare of the dentate gyrus. Top ...read more
Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] - No colocalization of Shank immunoreactivity with VGLUT2 puncta in the VGLUT2-band of the stratum pyramidale nor in the mossy fibers of ...read more
Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] - Colocalization of Shank immunoreactivity with VGLUT1 in the stratum radiatum/stratum lucidum of the CA3 region. Top box: Schematic ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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SHANK1 Antibody - BSA Free Summary

Immunogen
residues [SGPIYPGLFDIRSS] of the C-terminus of the SHANK1 protein.
Specificity
Reacts with residues [SGPIYPGLFDIRSS] of the C-terminus of the SHANK1 protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SHANK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:800
  • Immunohistochemistry 1:250-1:750
  • Immunohistochemistry-Paraffin 1:250-1:750
  • Western Blot 1:500-1:1000
Application Notes
This antibody is useful for Western blot, immunohistochemistry-Paraffin, and immunohistochemistry. The IF has been used at 1:800 on mouse G57B6
Publications
Read Publications using
NB300-167 in the following applications:

  • 3 publications
  • WB
    3 publications

Reactivity Notes

Human, rat and mouse. Other species have not been tested.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SHANK1 Antibody - BSA Free

  • SH3 and multiple ankyrin repeat domains 1
  • SH3 and multiple ankyrin repeat domains protein 1
  • Shank1
  • Somatostatin receptor-interacting protein
  • SSTR-interacting protein
  • SSTRIPSPANK-1
  • synamon

Background

Shank proteins are a family of scaffold proteins identified through their interaction with a variety of membrane and cyto-plasmic proteins. Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Shank proteins are crucial in receptor tyrosine kinase signaling. Recent studies suggest that disruption of glutamate receptors at the Shank-postsynaptic platform could contribute to the destruction of post-synaptic density which underlies synaptic dysfunction and loss in Alzheimers disease. Shank1 might be relevant to human autism spectrum disorders due to its differential role in specific cognitive processes along with its importance in synapse structure and function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB300-167
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC

Publications for SHANK1 Antibody (NB300-167)(7)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(3)
WB
(3)
All Applications
Filter By Species
Mouse
(5)
Rat
(2)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB300-167 Applications Species
Baczyk M, Alami NO, Delestree N et al. Synaptic restoration by cAMP/PKA drives activity-dependent neuroprotection to motoneurons in ALS J. Exp. Med. 2020-08-03 [PMID: 32484501] (ICC/IF, Mouse) ICC/IF Mouse
Dong Z, Chen W, Chen C et al. CUL3 Deficiency Causes Social Deficits and Anxiety-like Behaviors by Impairing Excitation-Inhibition Balance through the Promotion of Cap-Dependent Translation Neuron 2019-11-21 [PMID: 31780330]
Wolstenholme JT, Drobna Z, Henriksen AD et al. Transgenerational Bisphenol A causes deficits in social recognition and alters post-synaptic density genes in mice Endocrinology 2019-06-12 [PMID: 31188430] (Mouse) Mouse
Kerrisk Campbell M, Sheng M. USP8 Deubiquitinates SHANK3 to Control Synapse Density and SHANK3 Activity-dependent Protein Levels J. Neurosci. 2018-05-07 [PMID: 29735556] (WB, Mouse) WB Mouse
Heise C, Schroeder JC, Schoen M et al. Selective Localization of Shanks to VGLUT1-Positive Excitatory Synapses in the Mouse Hippocampus. Front Cell Neurosci. 2016-05-20 [PMID: 27199660] (ICC/IF, WB, Rat, Mouse) ICC/IF, WB Rat, Mouse
Hagmeyer S, Mangus K, Boeckers TM. Effects of Trace Metal Profiles Characteristic for Autism on Synapses in Cultured Neurons Neural Plasticity. 2015-02-19 [PMID: 25802764] (ICC/IF, Rat)

Details:
SHANK1 antibody was used for ICC-IF staining of Rat brain cultured hippocampal neurons grown under 3 different conditions (i) control cells/Ctrl grown in Neurobasal medium (ii) ASD1 cells grown in Neurobasal medium with addition of putative toxic metals 0.5 uM Cd, Cu, Hg, and 2uM Pb (iii) ASD2 cells grown in trace metal depleted Neurobasal medium that was reconstituted only for Mg and Ca, with addition of the putative toxic metals as mentioned for ASD1 conditions. For ICC-IF assay, the cells were - fixed in 4% PFA/1.5% sucrose/PBS at 4C for 20 minutes, permeablized 2X5 minutes with PBS having 0.2% Triton X-100 which followed blocking with 10% FBS/PBS for 1 hour at RT, incubated with primary antibody for 2 hours at RT followed by 1 hour incubation with Alexa conjugated secondary antibody and counterstained with DAPI (Figure 3).
ICC/IF Rat
Schmeisser MJ, Ey E, Wegener S, Bockmann J et al. Autistic-like behaviours and hyperactivity in mice lacking ProSAP1/Shank2. Nature 2012-01-01 [PMID: 22699619] (WB, Mouse) WB Mouse

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Product General Protocols

View specific protocols for SHANK1 Antibody (NB300-167): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SHANK1
Entrez