Novus Biologicals products are now on bio-techne.com

SH3MD2 Antibody - BSA Free

Images

 
Western Blot: SH3MD2 Antibody [NBP2-20347] - Sample (30 ug of whole cell lysate) A: U87-MG 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: SH3MD2 Antibody [NBP2-20347] - Immunohistochemical analysis of paraffin-embedded Normal Colon, using antibody at 1:250 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

Order Details

SH3MD2 Antibody - BSA Free Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SH3MD2. The exact sequence is proprietary.
Localization
Cytoplasm; perinuclear region (By similarity); Cell projection; lamellipodium; Golgi apparatus; trans-Golgi network
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SH3RF1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
112 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (80%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for SH3MD2 Antibody - BSA Free

  • EC 6.3.2
  • EC 6.3.2.-
  • KIAA1494SH3 multiple domains protein 2
  • Plenty of SH3s
  • POSHFLJ21602
  • Protein POSH
  • putative E3 ubiquitin-protein ligase SH3RF1
  • RING finger protein 142
  • RNF142plenty of SH3 domains
  • SH3 domain containing ring finger 1
  • SH3 domain-containing RING finger protein 1
  • SH3 multiple domains 2
  • SH3MD2

Background

This gene encodes a protein containing an N-terminus RING-finger, four SH3 domains, and a region implicated in binding of the Rho GTPase Rac. Via the RING-finger, the encoded protein has been shown to function as an ubiquitin-protein ligase involved in protein sorting at the trans-Golgi network. The encoded protein may also act as a scaffold for the c-Jun N-terminal kinase signaling pathway, facilitating the formation of a functional signaling module. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-48284
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-72710
Species: Ca, Hu, Mu, Rt
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P, IP, WB
MAB8930
Species: Hu
Applications: ICC, Simple Western, WB
AF4559
Species: Hu
Applications: WB
NBP1-81272
Species: Hu
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NB100-91266
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP2-13304
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-67895
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, IP, WB
NBP1-90201
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, Simple Western, WB
NBP2-21037
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-31361
Species: Hu
Applications: IHC, IHC-P, WB
NBP1-81988
Species: Hu
Applications: IHC, IHC-P
NBP1-82874
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P
NBP2-01109
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-89150
Species: Hu, Rt
Applications: IHC, IHC-P, WB
H00011026-M01
Species: Hu
Applications: ELISA, WB
MAB1846
Species: Hu, Mu, Rt
Applications: ICC, KO, WB
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for SH3MD2 Antibody (NBP2-20347) (0)

There are no publications for SH3MD2 Antibody (NBP2-20347).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SH3MD2 Antibody (NBP2-20347) (0)

There are no reviews for SH3MD2 Antibody (NBP2-20347). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SH3MD2 Antibody (NBP2-20347) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional SH3MD2 Products

Research Areas for SH3MD2 Antibody (NBP2-20347)

Find related products by research area.

Blogs on SH3MD2

There are no specific blogs for SH3MD2, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SH3MD2 Antibody - BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol SH3RF1
Uniprot