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PRPF8 Antibody - BSA Free

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Western Blot: PRPF8 Antibody [NBP2-93689] - Analysis of extracts of various cell lines, using PRPF8 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per ...read more
Immunocytochemistry/ Immunofluorescence: PRPF8 Antibody [NBP2-93689] - Analysis of U-251MG cells using PRPF8 . Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: PRPF8 Antibody [NBP2-93689] - Immunohistochemistry of paraffin-embedded rat pancreas using PRPF8 Rabbit pAb (NBP2-93689) at dilution of 1:200 (40x lens). Perform high pressure antigen ...read more
Immunohistochemistry-Paraffin: PRPF8 Antibody [NBP2-93689] - Immunohistochemistry of paraffin-embedded human colon carcinoma using PRPF8 Rabbit pAb (NBP2-93689) at dilution of 1:200 (40x lens). Perform high pressure ...read more
Immunohistochemistry-Paraffin: PRPF8 Antibody [NBP2-93689] - Immunohistochemistry of paraffin-embedded mouse brain using PRPF8 Rabbit pAb (NBP2-93689) at dilution of 1:200 (40x lens). Perform high pressure antigen ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

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PRPF8 Antibody - BSA Free Summary

Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PRPF8 (NP_006436.3). MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRPF8
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:50-1:100
  • Western Blot 1:500-1:1000
Theoretical MW
273 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for PRPF8 Antibody - BSA Free

  • apoptosis-regulated protein 1
  • apoptosis-regulated protein 2
  • p220
  • precursor mRNA processing protein
  • pre-mRNA-processing-splicing factor 8
  • PRP8 homolog
  • PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae)
  • PRP8 pre-mRNA processing factor 8 homolog (yeast)
  • Prp8
  • PRP8,220 kDa U5 snRNP-specific protein
  • PRPC8HPRP8
  • RP13
  • Splicing factor Prp8
  • U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p

Background

Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional PRPF8 Products

Array NBP2-93689

Research Areas for PRPF8 Antibody (NBP2-93689)

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Blogs on PRPF8

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Bioinformatics

Gene Symbol PRPF8