![Western Blot: SEC62 Antibody (PSH0-91) [NBP3-32940] - Western blot analysis of SEC62 on different lysates with Rabbit anti-SEC62 antibody (NBP3-32940) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: SH-SY5Y cell lysate (20 ug/Lane) Lane 4: HepG2 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: MCF7 cell lysate (20 ug/Lane) Lane 8: Daudi cell lysate (20 ug/Lane) Lane 9: PC-12 cell lysate (20 ug/Lane) Lane 10: Mouse liver tissue lysate (40 ug/Lane) Lane 11: Mouse brain tissue lysate (40 ug/Lane) Lane 12: Rat spleen tissue lysate (40 ug/Lane) Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32940) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32940_rabbit-sec62-mab-psh0-91-288202412461647.jpg "Western Blot: SEC62 Antibody (PSH0-91) [NBP3-32940] - Western blot analysis of SEC62 on different lysates with Rabbit anti-SEC62 antibody (NBP3-32940) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: SH-SY5Y cell lysate (20 ug/Lane) Lane 4: HepG2 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: MCF7 cell lysate (20 ug/Lane) Lane 8: Daudi cell lysate (20 ug/Lane) Lane 9: PC-12 cell lysate (20 ug/Lane) Lane 10: Mouse liver tissue lysate (40 ug/Lane) Lane 11: Mouse brain tissue lysate (40 ug/Lane) Lane 12: Rat spleen tissue lysate (40 ug/Lane) Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32940) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB |
| Clone | PSH0-91 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Synthetic peptide within human SEC62 aa 71-120 / 399. (Uniprot: Q99442) |
| Localization | Endoplasmic reticulum membrane. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | SEC62 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 46 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH7.4), 0.1% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for SEC62 Antibody (NBP3-32940)Find related products by research area.
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| Gene Symbol | SEC62 |
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