Reactivity | HuSpecies Glossary |
Applications | PAGE, Bioactivity |
Format | Carrier-Free |
Concentration | LYOPH |
Description | A single non-glycosylated polypeptide chain containing 164 amino acids corresponding to SCF/c-kit Ligand Source: E. coli Uniprot ID: P21583 Amino Acid Sequence: EGICRNRVTN NVKDVTKLVA NLPKDYMITL KYVPGMDVLP SHCWISEMVV QLSDSLTDLL DKFSNISEGL SNYSIIDKLV NIVDDLVECV KENSSKDLKK SFKSPEPRLF TPEEFFRIFN RSIDAFKDFV VASETSDCVV SSTLSPEKDS RVSVTKPFML PPVA |
Details of Functionality | SCF Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 x 10^5 IU/mg. |
Source | E. coli |
Protein/Peptide Type | Recombinant Protein |
Gene | KITLG |
Purity | > 97 % pure by SDS-PAGE and HPLC |
Endotoxin Note | Less than 1 EU/ug of SCF/c-kit Ligand as determined by LAL method. |
Dilutions |
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Theoretical MW | 18.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Buffer | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
Preservative | No Preservative |
Concentration | LYOPH |
Purity | > 97 % pure by SDS-PAGE and HPLC |
Reconstitution Instructions | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. |
Publications using NBP2-35256 | Applications | Species |
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Wang Y, Zhao Y, Liu L et al. Inhibitory effects of imatinib mesylate on human epidermal melanocytes. Clin Exp Dermatol 2014-03-01 [PMID: 24479586] | ||
Stadelmann L Erythropoietin receptor protects lung cancer cells from chemo-and radiotherapy independent of erythropoietin Thesis 2020-01-01 (KO) | KO |
Research Areas for SCF/c-kit Ligand Recombinant Protein (NBP2-35256)Find related products by research area.
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