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Recombinant Mouse SCF/c-kit Ligand Protein

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SDS-Page: Recombinant Mouse SCF/c-kit Ligand Protein [NBP2-35150] - SDS-PAGE of NBP2-35150
SDS-Page: Recombinant Mouse SCF/c-kit Ligand Protein [NBP2-35150] - SDS-PAGE of NBP2-35150

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier-Free
NBP2-35150-10ug
Carrier-Free
NBP2-35150-1mg
Carrier-Free
NBP2-35150-500ug
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Recombinant Mouse SCF/c-kit Ligand Protein Summary

Description
A single non-glycosylated polypeptide chain containing 165 amino acids corresponding to SCF/c-kit Ligand Source: E. coli

Uniprot ID: P20826

Amino Acid Sequence: MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA
Details of Functionality
SCF/c-kit Ligand protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferationassay using human TF-1 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 x 10^5 IU/mg.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
KITLG
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of SCF/c-kit Ligand as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Flow Cytometry
  • SDS-Page
Application Notes
Use in Flow reported in scientific literature (PMID:35874724).
Theoretical MW
18.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-35150 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative
No Preservative
Concentration
LYOPH
Purity
> 97 % pure by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Recombinant Mouse SCF/c-kit Ligand Protein

  • c-kit Ligand
  • DCUA
  • DFNA69
  • DKFZp686F2250
  • familial progressive hyperpigmentation 2
  • FPH2
  • FPHH
  • KIT ligand
  • Kitl
  • KITLG
  • KL-1
  • Mast cell growth factor
  • MGF
  • MGFSHEP7
  • SCF
  • SCFStem cell factor
  • SFc-Kit ligand
  • SHEP7
  • SLF
  • steel factor

Background

Stem Cell Factor (SCF) which binds to the c-Kit receptor is produced by fibroblasts and endothelial cells. The soluble and transmembrane forms of the protein are formed by alternative splicing of the same RNA transcript and the presence of both soluble and transmembrane It is required for normal hematopoietic function and plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. It also promotes mast cell adhesion, migration, proliferation, and survival.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for SCF/c-kit Ligand Recombinant Protein (NBP2-35150)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: FLOW.


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Bioinformatics

Gene Symbol KITLG
Uniprot