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S100A9 Antibody (MAC387) [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe, Gp, Gt, Pm, Pm, RbSpecies Glossary
Applications Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF
Clone
MAC387
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47667AF350
Alexa Fluor 405
NBP2-47667AF405
Alexa Fluor 488
NBP2-47667AF488
Alexa Fluor 532
NBP2-47667AF532
Alexa Fluor 594
NBP2-47667AF594
Alexa Fluor 647
NBP2-47667AF647
Alexa Fluor 700
NBP2-47667AF700
Alexa Fluor 750
NBP2-47667AF750
Allophycocyanin
NBP2-47667APC
Biotin
NBP2-47667B
DyLight 350
NBP2-47667UV
DyLight 405
NBP2-47667V
DyLight 488
NBP2-47667G
DyLight 550
NBP2-47667R
DyLight 594
NBP2-47667DL594
DyLight 650
NBP2-47667C
DyLight 680
NBP2-47667FR
DyLight 755
NBP2-47667IR
FITC
NBP2-47667F
HRP
NBP2-47667H
Janelia Fluor 525
NBP2-47667JF525
Janelia Fluor 549
NBP2-47667JF549
Janelia Fluor 646
NBP2-47667JF646
Janelia Fluor 669
NBP2-47667JF669
PE
NBP2-47667PE
PerCP
NBP2-47667PCP
Unconjugated
NBP2-45295-0.1mg
mFluor Violet 450 SE
NBP2-47667MFV450
mFluor Violet 500 SE
NBP2-47667MFV500
mFluor Violet 610 SE
NBP2-47667MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-45295-0.1mg
Azide and BSA Free
NBP2-47667-0.1mg
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S100A9 Antibody (MAC387) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Affinity Purified monocyte membrane preparation
Localization
Cytoplasmic
Specificity
Recognizes the L1 or Calprotectin molecule (S100A9), an intra-cytoplasmic antigen comprising of a 12kDa alpha chain and a 14kDa beta chain expressed by granulocytes, monocytes and by tissue macrophages. This monoclonal antibody reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
S100A9
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for S100A9 Antibody (MAC387) [CoraFluor™ 1]

  • CAGBMigration inhibitory factor-related protein 14
  • Calgranulin B
  • calgranulin-B
  • Calprotectin L1H subunit
  • CFAGMRP-14,60B8AG
  • CGLB
  • L1AG
  • LIAG
  • MAC387
  • MIF
  • MRP-14
  • MRP14Leukocyte L1 complex heavy chain
  • NIF
  • P14
  • protein S100-A9
  • S100 calcium binding protein A9 (calgranulin B)
  • S100 calcium binding protein A9
  • S100 calcium-binding protein A9 (calgranulin B)
  • S100 calcium-binding protein A9
  • S100A9

Background

S100A9 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A9 may function in the inhibition of casein kinase, and altered expression is associated with the disease cystic fibrosis. Overexpression of S100A9 has also recently been linked to a poor prognosis with invasive ductal carcinoma of the breast.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IL-6 [HRP]
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S100A9 Antibody
(MAC387) [CoraFluor&# ...
NBP2-47667CL1
S100A9 Antibody (MAC387) [CoraFluor™ 1]
Species: Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe, Gp, Gt, Pm, Pm, Rb
Applications: Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for S100A9 Antibody (NBP2-47667CL1). (Showing 1 - 2 of 2 FAQ).

  1. Have any of your S100A9 antibodies been tested for use in neutralization, or blocking assays, on human samples?
    • Unfortunately at this time we have not tested, or received customer feedback on our S100A9 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our S100A9 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional <a href="http://www.novusbio.com/support/innovators-reward.html" target="_self">Innovators Reward Program</a> information can be found using this link.
  2. We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.
    • Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol S100A9