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S100A8 Antibody (63N13G5) [Alexa Fluor® 700]

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Mouse splenocytes were fixed and permeabilized with Foxp3 Fixation/Permeabilization kit. Next, they were incubated with Fc block for 5 mins at RT and then stained with 1:100 dilution of this antibody for 25 mins at RT. Cells were acquired on a CytoFlex LX and analyzed using FlowJo 10. Image from verified customer review
Mouse splenocytes were fixed and permeabilized with Foxp3 Fixation/Permeabilization kit. Next, they were incubated with Fc block for 5 mins at RT and then stained with 1:100 dilution of this antibody for 25 mins at RT. ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC
Clone
63N13G5
Clonality
Monoclonal
Host
Rat
Conjugate
Alexa Fluor 700

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-27067AF350
Alexa Fluor 405
NBP2-27067AF405
Alexa Fluor 488
NBP2-27067AF488
Alexa Fluor 532
NBP2-27067AF532
Alexa Fluor 594
NBP2-27067AF594
Alexa Fluor 647
NBP2-27067AF647
Alexa Fluor 750
NBP2-27067AF750
Biotin
NBP2-27067B
DyLight 350
NBP2-27067UV
DyLight 405
NBP2-27067V
DyLight 488
NBP2-27067G
DyLight 550
NBP2-27067R
DyLight 594
NBP2-27067DL594
DyLight 650
NBP2-27067C
DyLight 680
NBP2-27067FR
DyLight 755
NBP2-27067IR
FITC
NBP2-25273F
HRP
NBP2-27067H
Janelia Fluor 549
NBP2-25273JF549
Janelia Fluor 646
NBP2-25273JF646
PE
NBP2-27067PE
Unconjugated
NBP2-27067
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-27067
Azide Free
NBP2-25273
BSA Free
NBP2-27067
BSA Free
NBP2-27067SS
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S100A8 Antibody (63N13G5) [Alexa Fluor® 700] Summary

Immunogen
Within the range of amino acids 2-98 of mouse S100A8 were used as the immunogen for the antibody.
Isotype
IgG2b Lambda
Clonality
Monoclonal
Host
Rat
Gene
S100A8
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-27067AF700 in the following applications:
Publications
Read Publication using
NBP2-27067AF700 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: rat S100A8 (81%).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for S100A8 Antibody (63N13G5) [Alexa Fluor® 700]

  • 60B8AG
  • CAGACP-10
  • Calgranulin A
  • calgranulin-A
  • Calprotectin L1L subunit
  • CFAG
  • CFAGL1Ag
  • CGLA
  • Cystic fibrosis antigen
  • Leukocyte L1 complex light chain
  • MA387
  • MIF
  • Migration inhibitory factor-related protein 8
  • MRP8
  • MRP-8
  • MRP8S100 calcium binding protein A8 (calgranulin A)
  • NIF
  • P8
  • protein S100-A8
  • S100 calcium binding protein A8
  • S100 calcium-binding protein A8 (calgranulin A)
  • S100 calcium-binding protein A8
  • S100A8
  • Urinary stone protein band A

Background

MRP8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for S100A8 Antibody (NBP2-27067AF700)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: FLOW.


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Review for S100A8 Antibody (NBP2-27067AF700) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-27067AF700:
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Images Ratings Applications Species Date Details
Flow Cytometry S100A8 NBP2-27067AF700
Enlarge 		5
reviewed by:
Mahesh Raundhal
 Flow Mouse 01/28/2018
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Summary

ApplicationFlow Cytometry
Sample TestedMouse splenocytes
SpeciesMouse
LotA23099

Comments

CommentsMouse splenocytes were fixed and permeabilized with Foxp3 Fixation/Permeabilization kit. Next, they were incubated with Fc block for 5 mins at RT and then stained with 1:100 dilution of this antibody for 25 mins at RT. Cells were acquired on a CytoFlex LX and analyzed using FlowJo 10.

Product General Protocols

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FAQs for S100A8 Antibody (NBP2-27067AF700). (Showing 1 - 2 of 2 FAQ).

  1. Have any of your MRP8 antibodies been tested for use in neutralization, or blocking assays, on human samples?
    • Unfortunately at this time we have not tested, or received customer feedback on our MMP8 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our MMP8 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional Innovators Reward Program information can be found using this link.
  2. We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.
    • Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.

Secondary Antibodies

 

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Recent Reviews

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Flow Cytometry S100A8 NBP2-27067AF700
Enlarge
 Mahesh Raundhal
01/28/2018
Application: Flow
Species: Mouse
read this review

Bioinformatics

Gene Symbol S100A8