![Western Blot: S100A4 Antibody (S100A4/1482) - Azide and BSA Free [NBP2-54400] - Western Blot Analysis of HeLa cell lysate using S100A4 Antibody (S100A4/1482).](http://images.novusbio.com/fullsize/S100A4-Antibody-S100A4-1482-Azide-and-BSA-Free-Western-Blot-NBP2-54400-img0007.jpg "Western Blot: S100A4 Antibody (S100A4/1482) - Azide and BSA Free [NBP2-54400] - Western Blot Analysis of HeLa cell lysate using S100A4 Antibody (S100A4/1482).")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | S100A4/1482 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53270). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous. |
| Immunogen | Recombinant fragment of human S100A4 protein (aa1-200) (exact sequence is proprietary) (Uniprot: P26447) |
| Localization | Cytoplasmic and Nuclear |
| Marker | Marker of Tumor Metastasis |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | S100A4 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_15885.jpg)
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Secondary Antibodies |
Isotype Controls |
Research Areas for S100A4 Antibody (NBP2-54400)Find related products by research area.
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Epithelial-Mesenchymal Transition (EMT) Markers Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a... Read full blog post. |
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| Gene Symbol | S100A4 |
![Immunocytochemistry/Immunofluorescence: S100A4 Antibody (S100A4/1482) - Azide and BSA Free [NBP2-54400] - Confocal immunofluorescence image of HeLa Cells using S100A4 Mouse Monoclonal Antibody (S100A4/1482) followed by goat anti-mouse IgG-CF488 (green). Reddot is used to label the nuclei Red.](http://images.novusbio.com/fullsize/S100A4-Antibody-S100A4-1482-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-54400-img0008.jpg "Immunocytochemistry/Immunofluorescence: S100A4 Antibody (S100A4/1482) - Azide and BSA Free [NBP2-54400] - Confocal immunofluorescence image of HeLa Cells using S100A4 Mouse Monoclonal Antibody (S100A4/1482) followed by goat anti-mouse IgG-CF488 (green). Reddot is used to label the nuclei Red.")
![Immunohistochemistry-Paraffin: S100A4 Antibody (S100A4/1482) - Azide and BSA Free [NBP2-54400] - Formalin--paraffin human Placenta stained with S100A4 Monoclonal Antibody (S100A4/1482).](http://images.novusbio.com/fullsize/S100A4-Antibody-S100A4-1482-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54400-img0001.jpg "Immunohistochemistry-Paraffin: S100A4 Antibody (S100A4/1482) - Azide and BSA Free [NBP2-54400] - Formalin--paraffin human Placenta stained with S100A4 Monoclonal Antibody (S100A4/1482).")
![Flow (Intracellular): S100A4 Antibody (S100A4/1482) - Azide and BSA Free [NBP2-54400] - An intracellular stain was performed on HeLa cells with S100A4 Antibody (S100A4/1482) NBP2-54400F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC Image using the Azide and BSA Free form of this antibody.](http://images.novusbio.com/fullsize/S100A4-Antibody-S100A4-1482-Azide-and-BSA-Free-Flow-Intracellular-NBP2-54400-img0006.jpg "Flow (Intracellular): S100A4 Antibody (S100A4/1482) - Azide and BSA Free [NBP2-54400] - An intracellular stain was performed on HeLa cells with S100A4 Antibody (S100A4/1482) NBP2-54400F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC Image using the Azide and BSA Free form of this antibody.")
![Western Blot: S100A4 Antibody (S100A4/1482) - Azide and BSA Free [NBP2-54400] - HeLa, A549 and A375 Cell Lysate using S100A4 Monoclonal Antibody (S100A4/1482).](http://images.novusbio.com/fullsize/S100A4-Antibody-S100A4-1482-Azide-and-BSA-Free-Western-Blot-NBP2-54400-img0002.jpg "Western Blot: S100A4 Antibody (S100A4/1482) - Azide and BSA Free [NBP2-54400] - HeLa, A549 and A375 Cell Lysate using S100A4 Monoclonal Antibody (S100A4/1482).")
![Flow Cytometry: S100A4 Antibody (S100A4/1482) - Azide and BSA Free [NBP2-54400] - Flow Cytometric Analysis of T98G cells using S100A4 Antibody (S100A4/1482) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/S100A4-Antibody-S100A4-1482-Azide-and-BSA-Free-Flow-Cytometry-NBP2-54400-img0005.jpg "Flow Cytometry: S100A4 Antibody (S100A4/1482) - Azide and BSA Free [NBP2-54400] - Flow Cytometric Analysis of T98G cells using S100A4 Antibody (S100A4/1482) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")